Expression of an N-methyl-D-aspartate-type receptor by human and rat osteoblasts and osteoclasts suggests a novel glutamate signaling pathway in bone

A J Patton; P G Genever; M A Birch; L J Suva; T M Skerry

Expression of an N-methyl-D-aspartate-type receptor by human and rat osteoblasts and osteoclasts suggests a novel glutamate signaling pathway in bone

A J Patton, P G Genever, M A Birch, L J Suva, T M Skerry

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Signaling between the various types of cells found in bone is responsible for controlling the activity of osteoblasts and osteoclasts, and therefore the regulation of bone mass. Our identification of a neuronal glutamate transporter in osteoblasts and osteocytes suggests the possibility that bone cells may use the excitatory amino acid glutamate as a signaling molecule. In these studies we report the expression of different subtypes of glutamate receptors in osteoblasts and osteoclasts in vitro and in vivo. We have identified expression in human and rat bone cells of N-methyl-D-aspartate receptor-1 (NMDAR-1) and to subunits and PSD-95, the NR IDA receptor clustering protein associated with signaling in the central nervous system. In situ hybridization and immunohistochemistry localized NMDAR-1 expression to osteoblasts and osteoclasts in human tissue sections, These findings strengthen the suggestion that glutamate is involved in signaling between bone cells. (C) 1998 by Elsevier Science Inc. All rights reserved.

Original language	English
Pages (from-to)	645-649
Number of pages	5
Journal	Bone
Volume	22
Issue number	6
Publication status	Published - Jun 1998

Keywords

osteoporosis
glutamate receptor
NMDA
bone formation
bone resorption

Cite this

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title = "Expression of an N-methyl-D-aspartate-type receptor by human and rat osteoblasts and osteoclasts suggests a novel glutamate signaling pathway in bone",

abstract = "Signaling between the various types of cells found in bone is responsible for controlling the activity of osteoblasts and osteoclasts, and therefore the regulation of bone mass. Our identification of a neuronal glutamate transporter in osteoblasts and osteocytes suggests the possibility that bone cells may use the excitatory amino acid glutamate as a signaling molecule. In these studies we report the expression of different subtypes of glutamate receptors in osteoblasts and osteoclasts in vitro and in vivo. We have identified expression in human and rat bone cells of N-methyl-D-aspartate receptor-1 (NMDAR-1) and to subunits and PSD-95, the NR IDA receptor clustering protein associated with signaling in the central nervous system. In situ hybridization and immunohistochemistry localized NMDAR-1 expression to osteoblasts and osteoclasts in human tissue sections, These findings strengthen the suggestion that glutamate is involved in signaling between bone cells. (C) 1998 by Elsevier Science Inc. All rights reserved.",

keywords = "osteoporosis, glutamate receptor, NMDA, bone formation, bone resorption",

author = "Patton, {A J} and Genever, {P G} and Birch, {M A} and Suva, {L J} and Skerry, {T M}",

year = "1998",

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journal = "Bone",

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TY - JOUR

T1 - Expression of an N-methyl-D-aspartate-type receptor by human and rat osteoblasts and osteoclasts suggests a novel glutamate signaling pathway in bone

AU - Patton, A J

AU - Genever, P G

AU - Birch, M A

AU - Suva, L J

AU - Skerry, T M

PY - 1998/6

Y1 - 1998/6

N2 - Signaling between the various types of cells found in bone is responsible for controlling the activity of osteoblasts and osteoclasts, and therefore the regulation of bone mass. Our identification of a neuronal glutamate transporter in osteoblasts and osteocytes suggests the possibility that bone cells may use the excitatory amino acid glutamate as a signaling molecule. In these studies we report the expression of different subtypes of glutamate receptors in osteoblasts and osteoclasts in vitro and in vivo. We have identified expression in human and rat bone cells of N-methyl-D-aspartate receptor-1 (NMDAR-1) and to subunits and PSD-95, the NR IDA receptor clustering protein associated with signaling in the central nervous system. In situ hybridization and immunohistochemistry localized NMDAR-1 expression to osteoblasts and osteoclasts in human tissue sections, These findings strengthen the suggestion that glutamate is involved in signaling between bone cells. (C) 1998 by Elsevier Science Inc. All rights reserved.

AB - Signaling between the various types of cells found in bone is responsible for controlling the activity of osteoblasts and osteoclasts, and therefore the regulation of bone mass. Our identification of a neuronal glutamate transporter in osteoblasts and osteocytes suggests the possibility that bone cells may use the excitatory amino acid glutamate as a signaling molecule. In these studies we report the expression of different subtypes of glutamate receptors in osteoblasts and osteoclasts in vitro and in vivo. We have identified expression in human and rat bone cells of N-methyl-D-aspartate receptor-1 (NMDAR-1) and to subunits and PSD-95, the NR IDA receptor clustering protein associated with signaling in the central nervous system. In situ hybridization and immunohistochemistry localized NMDAR-1 expression to osteoblasts and osteoclasts in human tissue sections, These findings strengthen the suggestion that glutamate is involved in signaling between bone cells. (C) 1998 by Elsevier Science Inc. All rights reserved.

KW - osteoporosis

KW - glutamate receptor

KW - NMDA

KW - bone formation

KW - bone resorption

M3 - Article

SN - 8756-3282

VL - 22

SP - 645

EP - 649

JO - Bone

JF - Bone

IS - 6

ER -