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Expression of fungal acetyl xylan esterase in Arabidopsis thaliana improves saccharification of stem lignocellulose.

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Expression of fungal acetyl xylan esterase in Arabidopsis thaliana improves saccharification of stem lignocellulose. / Pawar, Prashant Mohan-Anupama; Derba-Maceluch, Marta; Chong, Sun-Li; Gomez, Leonardo Dario; Miedes, Eva; Ratke, Christine; Gaertner, Cyril; Mouille, Gregory; McQueen Mason, Simon John; Molina, Antonio; Sellstedt, Anita; Tenkanen, Maija; Mellerowicz, Ewa.

In: Plant biotechnology journal, Vol. doi:10.1111/pbi.12393, doi:10.1111/pbi.12393, 11.05.2015.

Research output: Contribution to journalArticlepeer-review

Harvard

Pawar, PM-A, Derba-Maceluch, M, Chong, S-L, Gomez, LD, Miedes, E, Ratke, C, Gaertner, C, Mouille, G, McQueen Mason, SJ, Molina, A, Sellstedt, A, Tenkanen, M & Mellerowicz, E 2015, 'Expression of fungal acetyl xylan esterase in Arabidopsis thaliana improves saccharification of stem lignocellulose.', Plant biotechnology journal, vol. doi:10.1111/pbi.12393, doi:10.1111/pbi.12393. https://doi.org/10.1111/pbi.12393

APA

Pawar, P. M-A., Derba-Maceluch, M., Chong, S-L., Gomez, L. D., Miedes, E., Ratke, C., Gaertner, C., Mouille, G., McQueen Mason, S. J., Molina, A., Sellstedt, A., Tenkanen, M., & Mellerowicz, E. (2015). Expression of fungal acetyl xylan esterase in Arabidopsis thaliana improves saccharification of stem lignocellulose. Plant biotechnology journal, doi:10.1111/pbi.12393, [doi:10.1111/pbi.12393]. https://doi.org/10.1111/pbi.12393

Vancouver

Pawar PM-A, Derba-Maceluch M, Chong S-L, Gomez LD, Miedes E, Ratke C et al. Expression of fungal acetyl xylan esterase in Arabidopsis thaliana improves saccharification of stem lignocellulose. Plant biotechnology journal. 2015 May 11;doi:10.1111/pbi.12393. doi:10.1111/pbi.12393. https://doi.org/10.1111/pbi.12393

Author

Pawar, Prashant Mohan-Anupama ; Derba-Maceluch, Marta ; Chong, Sun-Li ; Gomez, Leonardo Dario ; Miedes, Eva ; Ratke, Christine ; Gaertner, Cyril ; Mouille, Gregory ; McQueen Mason, Simon John ; Molina, Antonio ; Sellstedt, Anita ; Tenkanen, Maija ; Mellerowicz, Ewa. / Expression of fungal acetyl xylan esterase in Arabidopsis thaliana improves saccharification of stem lignocellulose. In: Plant biotechnology journal. 2015 ; Vol. doi:10.1111/pbi.12393.

Bibtex - Download

@article{2e525278332f4d3bba3cbe252a2c9a95,
title = "Expression of fungal acetyl xylan esterase in Arabidopsis thaliana improves saccharification of stem lignocellulose.",
abstract = "Cell wall hemicelluloses and pectins are O-acetylated at specific positions, but the significance of these substitutions is poorly understood. Using a transgenic approach, we investigated how reducing the extent of O-acetylation in xylan affects cell wall chemistry, plant performance and the recalcitrance of lignocellulose to saccharification. The Aspergillus niger acetyl xylan esterase AnAXE1 was expressed in Arabidopsis under the control of either the constitutively expressed 35S CAMV promoter or a woody-tissue-specific GT43B aspen promoter, and the protein was targeted to the apoplast by its native signal peptide, resulting in elevated acetyl esterase activity in soluble and wall-bound protein extracts and reduced xylan acetylation. No significant alterations in cell wall composition were observed in the transgenic lines, but their xylans were more easily digested by a β-1,4-endoxylanase, and more readily extracted by hot water, acids or alkali. Enzymatic saccharification of lignocellulose after hot water and alkali pretreatments produced up to 20% more reducing sugars in several lines. Fermentation by Trametes versicolor of tissue hydrolysates from the line with a 30% reduction in acetyl content yielded ~70% more ethanol compared with wild type. Plants expressing 35S:AnAXE1 and pGT43B:AnAXE1 developed normally and showed increased resistance to the biotrophic pathogen Hyaloperonospora arabidopsidis, probably due to constitutive activation of defence pathways. However, unintended changes in xyloglucan and pectin acetylation were only observed in 35S:AnAXE1-expressing plants. This study demonstrates that postsynthetic xylan deacetylation in woody tissues is a promising strategy for optimizing lignocellulosic biomass for biofuel production.",
author = "Pawar, {Prashant Mohan-Anupama} and Marta Derba-Maceluch and Sun-Li Chong and Gomez, {Leonardo Dario} and Eva Miedes and Christine Ratke and Cyril Gaertner and Gregory Mouille and {McQueen Mason}, {Simon John} and Antonio Molina and Anita Sellstedt and Maija Tenkanen and Ewa Mellerowicz",
year = "2015",
month = may,
day = "11",
doi = "10.1111/pbi.12393",
language = "English",
volume = "doi:10.1111/pbi.12393",
journal = "Plant biotechnology journal",
issn = "1467-7644",
publisher = "Wiley-Blackwell",

}

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TY - JOUR

T1 - Expression of fungal acetyl xylan esterase in Arabidopsis thaliana improves saccharification of stem lignocellulose.

AU - Pawar, Prashant Mohan-Anupama

AU - Derba-Maceluch, Marta

AU - Chong, Sun-Li

AU - Gomez, Leonardo Dario

AU - Miedes, Eva

AU - Ratke, Christine

AU - Gaertner, Cyril

AU - Mouille, Gregory

AU - McQueen Mason, Simon John

AU - Molina, Antonio

AU - Sellstedt, Anita

AU - Tenkanen, Maija

AU - Mellerowicz, Ewa

PY - 2015/5/11

Y1 - 2015/5/11

N2 - Cell wall hemicelluloses and pectins are O-acetylated at specific positions, but the significance of these substitutions is poorly understood. Using a transgenic approach, we investigated how reducing the extent of O-acetylation in xylan affects cell wall chemistry, plant performance and the recalcitrance of lignocellulose to saccharification. The Aspergillus niger acetyl xylan esterase AnAXE1 was expressed in Arabidopsis under the control of either the constitutively expressed 35S CAMV promoter or a woody-tissue-specific GT43B aspen promoter, and the protein was targeted to the apoplast by its native signal peptide, resulting in elevated acetyl esterase activity in soluble and wall-bound protein extracts and reduced xylan acetylation. No significant alterations in cell wall composition were observed in the transgenic lines, but their xylans were more easily digested by a β-1,4-endoxylanase, and more readily extracted by hot water, acids or alkali. Enzymatic saccharification of lignocellulose after hot water and alkali pretreatments produced up to 20% more reducing sugars in several lines. Fermentation by Trametes versicolor of tissue hydrolysates from the line with a 30% reduction in acetyl content yielded ~70% more ethanol compared with wild type. Plants expressing 35S:AnAXE1 and pGT43B:AnAXE1 developed normally and showed increased resistance to the biotrophic pathogen Hyaloperonospora arabidopsidis, probably due to constitutive activation of defence pathways. However, unintended changes in xyloglucan and pectin acetylation were only observed in 35S:AnAXE1-expressing plants. This study demonstrates that postsynthetic xylan deacetylation in woody tissues is a promising strategy for optimizing lignocellulosic biomass for biofuel production.

AB - Cell wall hemicelluloses and pectins are O-acetylated at specific positions, but the significance of these substitutions is poorly understood. Using a transgenic approach, we investigated how reducing the extent of O-acetylation in xylan affects cell wall chemistry, plant performance and the recalcitrance of lignocellulose to saccharification. The Aspergillus niger acetyl xylan esterase AnAXE1 was expressed in Arabidopsis under the control of either the constitutively expressed 35S CAMV promoter or a woody-tissue-specific GT43B aspen promoter, and the protein was targeted to the apoplast by its native signal peptide, resulting in elevated acetyl esterase activity in soluble and wall-bound protein extracts and reduced xylan acetylation. No significant alterations in cell wall composition were observed in the transgenic lines, but their xylans were more easily digested by a β-1,4-endoxylanase, and more readily extracted by hot water, acids or alkali. Enzymatic saccharification of lignocellulose after hot water and alkali pretreatments produced up to 20% more reducing sugars in several lines. Fermentation by Trametes versicolor of tissue hydrolysates from the line with a 30% reduction in acetyl content yielded ~70% more ethanol compared with wild type. Plants expressing 35S:AnAXE1 and pGT43B:AnAXE1 developed normally and showed increased resistance to the biotrophic pathogen Hyaloperonospora arabidopsidis, probably due to constitutive activation of defence pathways. However, unintended changes in xyloglucan and pectin acetylation were only observed in 35S:AnAXE1-expressing plants. This study demonstrates that postsynthetic xylan deacetylation in woody tissues is a promising strategy for optimizing lignocellulosic biomass for biofuel production.

U2 - 10.1111/pbi.12393

DO - 10.1111/pbi.12393

M3 - Article

VL - doi:10.1111/pbi.12393

JO - Plant biotechnology journal

JF - Plant biotechnology journal

SN - 1467-7644

M1 - doi:10.1111/pbi.12393

ER -