Extracellular regulation of developmental cell signaling by XtSulf1

TY - JOUR

T1 - Extracellular regulation of developmental cell signaling by XtSulf1

AU - Freeman, Stephen D.

AU - Moore, Wendy M.

AU - Guiral, Emily C.

AU - Holme, Alexandra D.

AU - Turnbull, Jeremy E.

AU - Pownall, Mary E.

PY - 2008/8/15

Y1 - 2008/8/15

N2 - Heparan Sulfate proteoglycans (HSPGs) are synthesised and modified in the Golgi before they are presented at the cell surface. Modifications include the addition of sulfate groups at specific positions on sugar residues along the heparan sulfate (HS) chain which results in a structural heterogeneity that underpins the ability of HSPGs to bind with high affinity to many different proteins, including growth factors and their receptors. Sulf1 codes for a 6-0-endosulfatase that is present and active extracellularly, providing a further mechanism to generate structural diversity through the post-synthetic remodelling of HS. Here we use Xenopus embryos to demonstrate in vivo that Xtsulf1 plays an important role in modulating cell signaling during development. We show that while XtSulf1 can enhance the axis-inducing activity of Wnt11, XtSulf1 acts during embryogenesis to restrict BMP and FGF signaling, (C) 2008 Elsevier Inc. All rights reserved.

AB - Heparan Sulfate proteoglycans (HSPGs) are synthesised and modified in the Golgi before they are presented at the cell surface. Modifications include the addition of sulfate groups at specific positions on sugar residues along the heparan sulfate (HS) chain which results in a structural heterogeneity that underpins the ability of HSPGs to bind with high affinity to many different proteins, including growth factors and their receptors. Sulf1 codes for a 6-0-endosulfatase that is present and active extracellularly, providing a further mechanism to generate structural diversity through the post-synthetic remodelling of HS. Here we use Xenopus embryos to demonstrate in vivo that Xtsulf1 plays an important role in modulating cell signaling during development. We show that while XtSulf1 can enhance the axis-inducing activity of Wnt11, XtSulf1 acts during embryogenesis to restrict BMP and FGF signaling, (C) 2008 Elsevier Inc. All rights reserved.

KW - FGF

KW - Wnt

KW - BMP

KW - Xenopus

KW - HSPGs

KW - sulfatase

KW - HEPARAN-SULFATE PROTEOGLYCANS

KW - CONVERGENT EXTENSION MOVEMENTS

KW - BONE MORPHOGENETIC PROTEIN-4

KW - GROWTH-FACTOR RECEPTOR

KW - XENOPUS-EMBRYOS

KW - NEURAL INDUCTION

KW - MESODERM INDUCTION

KW - RETINAL DEVELOPMENT

KW - AXIS FORMATION

KW - EARLY RESPONSE

UR - http://www.scopus.com/inward/record.url?scp=48749120918&partnerID=8YFLogxK

U2 - 10.1016/j.ydbio.2008.05.554

DO - 10.1016/j.ydbio.2008.05.554

M3 - Article

SN - 0012-1606

VL - 320

SP - 436

EP - 445

JO - Developmental Biology

JF - Developmental Biology

IS - 2

ER -