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Extraction of Ancient DNA from Plant Remains. / Wales, Nathan; Kistler, Logan.
Ancient DNA: Methods and Protocols. ed. / Beth Shapiro; Axel Barlow; Peter D. Heintzman; Michael Hofreiter; Johanna L. A. Paijmans. Vol. 1963 2nd. ed. 2019. p. 45-55 (Methods in molecular biology (Clifton, N.J.)).
Research output: Chapter in Book/Report/Conference proceeding › Chapter
Harvard
Wales, N & Kistler, L 2019,
Extraction of Ancient DNA from Plant Remains. in B Shapiro, A Barlow, PD Heintzman, M Hofreiter & JLA Paijmans (eds),
Ancient DNA: Methods and Protocols. 2nd edn, vol. 1963, Methods in molecular biology (Clifton, N.J.), pp. 45-55.
https://doi.org/10.1007/978-1-4939-9176-1_6
APA
Wales, N., & Kistler, L. (2019).
Extraction of Ancient DNA from Plant Remains. In B. Shapiro, A. Barlow, P. D. Heintzman, M. Hofreiter, & J. L. A. Paijmans (Eds.),
Ancient DNA: Methods and Protocols (2nd ed., Vol. 1963, pp. 45-55). (Methods in molecular biology (Clifton, N.J.)).
https://doi.org/10.1007/978-1-4939-9176-1_6
Vancouver
Wales N, Kistler L.
Extraction of Ancient DNA from Plant Remains. In Shapiro B, Barlow A, Heintzman PD, Hofreiter M, Paijmans JLA, editors, Ancient DNA: Methods and Protocols. 2nd ed. Vol. 1963. 2019. p. 45-55. (Methods in molecular biology (Clifton, N.J.)).
https://doi.org/10.1007/978-1-4939-9176-1_6
Author
Wales, Nathan ; Kistler, Logan. / Extraction of Ancient DNA from Plant Remains. Ancient DNA: Methods and Protocols. editor / Beth Shapiro ; Axel Barlow ; Peter D. Heintzman ; Michael Hofreiter ; Johanna L. A. Paijmans. Vol. 1963 2nd. ed. 2019. pp. 45-55 (Methods in molecular biology (Clifton, N.J.)).
@inbook{66e7512a68d042e195010184acc422c5,
title = "Extraction of Ancient DNA from Plant Remains",
abstract = "Ancient plant remains from archaeological sites, paleoenvironmental contexts, and herbaria provide excellent opportunities for interrogating plant genetics over Quaternary timescales using ancient DNA (aDNA)-based analyses. A variety of plant tissues, preserved primarily by desiccation and anaerobic waterlogging, have proven to be viable sources of aDNA. Plant tissues are anatomically and chemically diverse and therefore require optimized DNA extraction approaches. Here, we describe a plant DNA isolation protocol that performs well in most contexts. We include recommendations for optimization to retain the very short DNA fragments that are expected to be preserved in degraded tissues.",
keywords = "Ancient plant DNA, Archaeobotany, Archaeogenomics, DNA extraction, Paleoethnobotany",
author = "Nathan Wales and Logan Kistler",
year = "2019",
month = mar,
day = "16",
doi = "10.1007/978-1-4939-9176-1_6",
language = "English",
isbn = "9781493991754",
volume = "1963",
series = "Methods in molecular biology (Clifton, N.J.)",
pages = "45--55",
editor = "Beth Shapiro and Axel Barlow and Heintzman, {Peter D.} and Michael Hofreiter and Paijmans, {Johanna L. A.}",
booktitle = "Ancient DNA: Methods and Protocols",
edition = "2nd",
}
RIS (suitable for import to EndNote) - Download
TY - CHAP
T1 - Extraction of Ancient DNA from Plant Remains
AU - Wales, Nathan
AU - Kistler, Logan
PY - 2019/3/16
Y1 - 2019/3/16
N2 - Ancient plant remains from archaeological sites, paleoenvironmental contexts, and herbaria provide excellent opportunities for interrogating plant genetics over Quaternary timescales using ancient DNA (aDNA)-based analyses. A variety of plant tissues, preserved primarily by desiccation and anaerobic waterlogging, have proven to be viable sources of aDNA. Plant tissues are anatomically and chemically diverse and therefore require optimized DNA extraction approaches. Here, we describe a plant DNA isolation protocol that performs well in most contexts. We include recommendations for optimization to retain the very short DNA fragments that are expected to be preserved in degraded tissues.
AB - Ancient plant remains from archaeological sites, paleoenvironmental contexts, and herbaria provide excellent opportunities for interrogating plant genetics over Quaternary timescales using ancient DNA (aDNA)-based analyses. A variety of plant tissues, preserved primarily by desiccation and anaerobic waterlogging, have proven to be viable sources of aDNA. Plant tissues are anatomically and chemically diverse and therefore require optimized DNA extraction approaches. Here, we describe a plant DNA isolation protocol that performs well in most contexts. We include recommendations for optimization to retain the very short DNA fragments that are expected to be preserved in degraded tissues.
KW - Ancient plant DNA
KW - Archaeobotany
KW - Archaeogenomics
KW - DNA extraction
KW - Paleoethnobotany
UR - http://www.scopus.com/inward/record.url?scp=85062982841&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-9176-1_6
DO - 10.1007/978-1-4939-9176-1_6
M3 - Chapter
C2 - 30875043
AN - SCOPUS:85062982841
SN - 9781493991754
VL - 1963
T3 - Methods in molecular biology (Clifton, N.J.)
SP - 45
EP - 55
BT - Ancient DNA: Methods and Protocols
A2 - Shapiro, Beth
A2 - Barlow, Axel
A2 - Heintzman, Peter D.
A2 - Hofreiter, Michael
A2 - Paijmans, Johanna L. A.
ER -