Fabrication and Application of Isotopically Labelled Gold Arrays for Multiplexed Peptide Analysis

Roberto Castangia, Sian Hudson, Helen Robinson, Sabine Flitsch, Jane Thomas-Oates, Anne Routledge

Research output: Contribution to journalArticlepeer-review

Abstract

A new array-based technology for the simultaneous capture, chemical labelling and mass spectrometry analysis of peptides is presented. Isotopically labelled self-assembled monolayer (SAM) gold arrays are constructed and used simultaneously to capture and label a range of peptides. The array-immobilised, labelled peptides were released by MALDI ablation, analysed by MALDI mass spectrometry and readily identified as labelled peptides from their characteristic isotope pattern. This new solid-phase array platform has the advantage of minimal sample manipulation and is suitable for multiple analyses of single protein digests on a single MALDI target plate.

Original languageEnglish
Number of pages9
JournalChembiochem
Early online date12 Sep 2016
DOIs
Publication statusE-pub ahead of print - 12 Sep 2016

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