Fabrication and Application of Isotopically Labelled Gold Arrays for Multiplexed Peptide Analysis

Roberto Castangia; Sian Hudson; Helen Robinson; Sabine Flitsch; Jane Thomas-Oates; Anne Routledge

doi:10.1002/cbic.201600347

Fabrication and Application of Isotopically Labelled Gold Arrays for Multiplexed Peptide Analysis

Roberto Castangia, Sian Hudson, Helen Robinson, Sabine Flitsch, Jane Thomas-Oates, Anne Routledge

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

A new array-based technology for the simultaneous capture, chemical labelling and mass spectrometry analysis of peptides is presented. Isotopically labelled self-assembled monolayer (SAM) gold arrays are constructed and used simultaneously to capture and label a range of peptides. The array-immobilised, labelled peptides were released by MALDI ablation, analysed by MALDI mass spectrometry and readily identified as labelled peptides from their characteristic isotope pattern. This new solid-phase array platform has the advantage of minimal sample manipulation and is suitable for multiple analyses of single protein digests on a single MALDI target plate.

Original language	English
Number of pages	9
Journal	Chembiochem
Early online date	12 Sep 2016
DOIs	https://doi.org/10.1002/cbic.201600347
Publication status	E-pub ahead of print - 12 Sep 2016

Bibliographical note

© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details.

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10.1002/cbic.201600347

cphc201600848
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details.
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abstract = "A new array-based technology for the simultaneous capture, chemical labelling and mass spectrometry analysis of peptides is presented. Isotopically labelled self-assembled monolayer (SAM) gold arrays are constructed and used simultaneously to capture and label a range of peptides. The array-immobilised, labelled peptides were released by MALDI ablation, analysed by MALDI mass spectrometry and readily identified as labelled peptides from their characteristic isotope pattern. This new solid-phase array platform has the advantage of minimal sample manipulation and is suitable for multiple analyses of single protein digests on a single MALDI target plate.",

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AU - Flitsch, Sabine

AU - Thomas-Oates, Jane

AU - Routledge, Anne

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Fabrication and Application of Isotopically Labelled Gold Arrays for Multiplexed Peptide Analysis

Abstract

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