Fate mapping reveals origin and dynamics of lymph node follicular dendritic cells

Meryem Jarjour, Audrey Jorquera, Isabelle Mondor, Stephan Wienert, Priyanka Narang, Mark C. Coles, Frederick Klauschen, Marc Bajénoff

Research output: Contribution to journalArticlepeer-review


Follicular dendritic cells (FDCs) regulate B cell function and development of high affinity antibody responses but little is known about their biology. FDCs associate in intricate cellular networks within secondary lymphoid organs. In vitro and ex vivo methods, therefore, allow only limited understanding of the genuine immunobiology of FDCs in their native habitat. Herein, we used various multicolor fate mapping systems to investigate the ontogeny and dynamics of lymph node (LN) FDCs in situ. We show that LN FDC networks arise from the clonal expansion and differentiation of marginal reticular cells (MRCs), a population of lymphoid stromal cells lining the LN subcapsular sinus. We further demonstrate that during an immune response, FDCs accumulate in germinal centers and that neither the recruitment of circulating progenitors nor the division of local mature FDCs significantly contributes to this accumulation. Rather, we provide evidence that newly generated FDCs also arise from the proliferation and differentiation of MRCs, thus unraveling a critical function of this poorly defined stromal cell population.

Original languageEnglish
Pages (from-to)1109-1122
Number of pages14
JournalJournal of Experimental Medicine
Issue number6
Publication statusPublished - 26 May 2014

Bibliographical note

© 2014 Jarjour et al.

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