Fate mapping reveals origin and dynamics of lymph node follicular dendritic cells

TY - JOUR

T1 - Fate mapping reveals origin and dynamics of lymph node follicular dendritic cells

AU - Jarjour, Meryem

AU - Jorquera, Audrey

AU - Mondor, Isabelle

AU - Wienert, Stephan

AU - Narang, Priyanka

AU - Coles, Mark C.

AU - Klauschen, Frederick

AU - Bajénoff, Marc

N1 - © 2014 Jarjour et al.

PY - 2014/5/26

Y1 - 2014/5/26

N2 - Follicular dendritic cells (FDCs) regulate B cell function and development of high affinity antibody responses but little is known about their biology. FDCs associate in intricate cellular networks within secondary lymphoid organs. In vitro and ex vivo methods, therefore, allow only limited understanding of the genuine immunobiology of FDCs in their native habitat. Herein, we used various multicolor fate mapping systems to investigate the ontogeny and dynamics of lymph node (LN) FDCs in situ. We show that LN FDC networks arise from the clonal expansion and differentiation of marginal reticular cells (MRCs), a population of lymphoid stromal cells lining the LN subcapsular sinus. We further demonstrate that during an immune response, FDCs accumulate in germinal centers and that neither the recruitment of circulating progenitors nor the division of local mature FDCs significantly contributes to this accumulation. Rather, we provide evidence that newly generated FDCs also arise from the proliferation and differentiation of MRCs, thus unraveling a critical function of this poorly defined stromal cell population.

AB - Follicular dendritic cells (FDCs) regulate B cell function and development of high affinity antibody responses but little is known about their biology. FDCs associate in intricate cellular networks within secondary lymphoid organs. In vitro and ex vivo methods, therefore, allow only limited understanding of the genuine immunobiology of FDCs in their native habitat. Herein, we used various multicolor fate mapping systems to investigate the ontogeny and dynamics of lymph node (LN) FDCs in situ. We show that LN FDC networks arise from the clonal expansion and differentiation of marginal reticular cells (MRCs), a population of lymphoid stromal cells lining the LN subcapsular sinus. We further demonstrate that during an immune response, FDCs accumulate in germinal centers and that neither the recruitment of circulating progenitors nor the division of local mature FDCs significantly contributes to this accumulation. Rather, we provide evidence that newly generated FDCs also arise from the proliferation and differentiation of MRCs, thus unraveling a critical function of this poorly defined stromal cell population.

U2 - 10.1084/jem.20132409

DO - 10.1084/jem.20132409

M3 - Article

C2 - 24863064

SN - 0022-1007

VL - 211

SP - 1109

EP - 1122

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

IS - 6

ER -