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Fatty acid desaturases from the microalga Thalassiosira pseudonana

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JournalFEBS Journal
DatePublished - Jul 2005
Issue number13
Volume272
Number of pages12
Pages (from-to)3401-3412
Original languageEnglish

Abstract

Analysis of a draft nuclear genome sequence of the diatom Thalassiosira pseudonana revealed the presence of 11 open reading frames showing significant similarity to functionally characterized fatty acid front-end desaturases. The corresponding genes occupy discrete chromosomal locations as determined by comparison with the recently published genome sequence. Phylogenetic analysis showed that two of the T. pseudonana desaturase (Tpdes) sequences grouped with proteobacterial desaturases that lack a fused cytochrome b5 domain. Among the nine remaining gene sequences, temporal expression analysis revealed that seven were expressed in T. pseudonana cells. One of these, TpdesN, was previously characterized as encoding a Delta(11)-desaturase active on palmitic acid. From the six remaining putative desaturase genes, we report here that three, TpdesI, TpdesO and TpdesK, respectively encode Delta(6)-, Delta(5)- and Delta(4)-desaturases involved in production of the health beneficial polyunsaturated fatty acid DHA (docosahexaenoic acid). Furthermore, we show that one of the remaining genes, TpdesB, encodes a Delta(8)-sphingolipid desaturase with strong preference for dihydroxylated substrates.

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    Research areas

  • desaturases, long chain polyunsaturated fatty acids, sphingolipids, Thalassiosira pseudonana, yeast expression, DOCOSAHEXAENOIC ACID, EICOSAPENTAENOIC ACID, DELTA(8)-SPHINGOLIPID DESATURASE, SPHINGOLIPID DESATURASE, BORAGO-OFFICINALIS, EUGLENA-GRACILIS, IDENTIFICATION, BIOSYNTHESIS, PLANTS, RECONSTITUTION

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