FGF signalling modulates transcriptional repression by Xenopus groucho-related-4

TY - JOUR

T1 - FGF signalling modulates transcriptional repression by Xenopus groucho-related-4

AU - Burks, Patrick J.

AU - Isaacs, Harry V.

AU - Pownall, Mary E.

PY - 2009/5

Y1 - 2009/5

N2 - Background information. Developmental cell signals co-operate in the processes of cell specification and tissue patterning during embryogenesis. Interactions between the FGF (fibroblast growth factor) and Wnt signalling pathways have been demonstrated in a number of developmental processes, including mesoderm formation in amphibian embryos. However, the mechanism underlying the interactions between these key signalling pathways remains unclear.Results. In the present study, we find that the ability of TLE4/Xgrg4 (transducin-like enhancer of split 4/Xenopus groucho-related gene 4) to inhibit a transcriptional target of canonical Writ signalling is reduced in the presence of FGF and that this is partially dependent on a consensus site for MAPK (mitogen-activated protein kinase) phosphorylation in TLE4/Xgrg4.Conclusions. These data suggest to us a novel molecular mechanism where FGF and Wnt signalling pathways interact at the level of the co-repressor TLE4/Xgrg4: the weakening of TLE4/Xgrg4 repression by FGF signalling, combined with the stabilization of beta-catenin by Wnt signals, enhances expression of Wnt target genes.

AB - Background information. Developmental cell signals co-operate in the processes of cell specification and tissue patterning during embryogenesis. Interactions between the FGF (fibroblast growth factor) and Wnt signalling pathways have been demonstrated in a number of developmental processes, including mesoderm formation in amphibian embryos. However, the mechanism underlying the interactions between these key signalling pathways remains unclear.Results. In the present study, we find that the ability of TLE4/Xgrg4 (transducin-like enhancer of split 4/Xenopus groucho-related gene 4) to inhibit a transcriptional target of canonical Writ signalling is reduced in the presence of FGF and that this is partially dependent on a consensus site for MAPK (mitogen-activated protein kinase) phosphorylation in TLE4/Xgrg4.Conclusions. These data suggest to us a novel molecular mechanism where FGF and Wnt signalling pathways interact at the level of the co-repressor TLE4/Xgrg4: the weakening of TLE4/Xgrg4 repression by FGF signalling, combined with the stabilization of beta-catenin by Wnt signals, enhances expression of Wnt target genes.

KW - mesoderm

KW - mitogen-activated protein kinase (MAPK)

KW - myogenic determination factor (MyoD)

KW - signal transduction

KW - Wnt signalling

KW - FIBROBLAST-GROWTH-FACTOR

KW - MESODERM FORMATION

KW - BETA-CATENIN

KW - MYOD INDUCTION

KW - MAP KINASE

KW - EXPRESSION

KW - WNT

KW - EMBRYOS

KW - GENES

KW - EFGF

UR - http://www.scopus.com/inward/record.url?scp=64749088602&partnerID=8YFLogxK

U2 - 10.1042/BC20080136

DO - 10.1042/BC20080136

M3 - Article

SN - 0248-4900

VL - 101

SP - 301

EP - 308

JO - Biology of the Cell

JF - Biology of the Cell

IS - 5

ER -