First molecular detection of a viral pathogen in Ugandan honey bees

Robert Kajobe; Gay Marris; Giles Budge; Lynn Laurenson; Guido Cordoni; Ben Jones; Selwyn Wilkins; Andrew G S Cuthbertson; Mike A Brown

doi:10.1016/j.jip.2010.02.007

First molecular detection of a viral pathogen in Ugandan honey bees

Robert Kajobe, Gay Marris, Giles Budge, Lynn Laurenson, Guido Cordoni, Ben Jones, Selwyn Wilkins, Andrew G S Cuthbertson, Mike A Brown

Language and Linguistic Science

Research output: Contribution to journal › Article › peer-review

Abstract

Ugandan honey bees (Apis mellifera L.) produce honey, and are key pollinators within commercial crops and natural ecosystems. Real-time RT-PCR was used to screen immature and adult bees collected from 63 beekeeping sites across Uganda for seven viral pathogens. No samples tested positive for Chronic bee paralysis virus, Sacbrood virus, Deformed wing virus, Acute bee paralysis virus, Apis iridescent virus or Israeli acute paralysis virus. However, Black queen cell virus (BQCV) was found in 35.6% of samples. It occurred in adults and larvae, and was most prevalent in the Western highlands, accounting for over 40% of positive results nationally.

Original language	English
Pages (from-to)	153-6
Number of pages	4
Journal	Journal of invertebrate pathology
Volume	104
Issue number	2
DOIs	https://doi.org/10.1016/j.jip.2010.02.007
Publication status	Published - Jun 2010

Keywords

Animals
Bees
Insect Viruses
Reverse Transcriptase Polymerase Chain Reaction
Uganda
Virus Diseases

Access to Document

10.1016/j.jip.2010.02.007

Cite this

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title = "First molecular detection of a viral pathogen in Ugandan honey bees",

abstract = "Ugandan honey bees (Apis mellifera L.) produce honey, and are key pollinators within commercial crops and natural ecosystems. Real-time RT-PCR was used to screen immature and adult bees collected from 63 beekeeping sites across Uganda for seven viral pathogens. No samples tested positive for Chronic bee paralysis virus, Sacbrood virus, Deformed wing virus, Acute bee paralysis virus, Apis iridescent virus or Israeli acute paralysis virus. However, Black queen cell virus (BQCV) was found in 35.6% of samples. It occurred in adults and larvae, and was most prevalent in the Western highlands, accounting for over 40% of positive results nationally.",

keywords = "Animals, Bees, Insect Viruses, Reverse Transcriptase Polymerase Chain Reaction, Uganda, Virus Diseases",

author = "Robert Kajobe and Gay Marris and Giles Budge and Lynn Laurenson and Guido Cordoni and Ben Jones and Selwyn Wilkins and Cuthbertson, {Andrew G S} and Brown, {Mike A}",

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AU - Kajobe, Robert

AU - Marris, Gay

AU - Budge, Giles

AU - Laurenson, Lynn

AU - Cordoni, Guido

AU - Jones, Ben

AU - Wilkins, Selwyn

AU - Cuthbertson, Andrew G S

AU - Brown, Mike A

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AB - Ugandan honey bees (Apis mellifera L.) produce honey, and are key pollinators within commercial crops and natural ecosystems. Real-time RT-PCR was used to screen immature and adult bees collected from 63 beekeeping sites across Uganda for seven viral pathogens. No samples tested positive for Chronic bee paralysis virus, Sacbrood virus, Deformed wing virus, Acute bee paralysis virus, Apis iridescent virus or Israeli acute paralysis virus. However, Black queen cell virus (BQCV) was found in 35.6% of samples. It occurred in adults and larvae, and was most prevalent in the Western highlands, accounting for over 40% of positive results nationally.

KW - Animals

KW - Bees

KW - Insect Viruses

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Uganda

KW - Virus Diseases

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DO - 10.1016/j.jip.2010.02.007

M3 - Article

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SN - 0022-2011

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JO - Journal of invertebrate pathology

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