Flow cytometric methodology for the detection of de novo human T-cell leukemia virus -1 infection in vitro: a tool to study novel infection inhibitors

Carina Peres, Yuetsu Tanaka, Fabiola Martin, James Martin Fox

Research output: Contribution to journalArticlepeer-review

Abstract

Methodology to detect and study de novo human T-cell leukemia virus (HTLV)-1 infection is
required to further our knowledge of the viruses’ mechanisms of infection and to study
potential therapeutic interventions. Whilst methodology currently exists, utilisation of an anti-
Tax antibody to detect de novo Tax expression in permissive cells labelled with cell tracker
allowing for the detection by flow cytometry of new infection after co-culture with donor cell
lines productively infected with HTLV-1 is an alternative strategy. Using this methodology,
we have been able to detect de novo infection of the T cell line HUT78 following co-culture
with the productively infected HTLV-1 donor cell line MT-2 and to confirm that infection
can be effectively blocked with well characterised infection inhibitors. This methodology will
benefit experimental studies examining HTLV infection in vitro and may aid identification of
therapeutic agents that block this process.
Original languageEnglish
Article number113728
JournalJournal of virological methods
Volume274
Early online date8 Sept 2019
Publication statusE-pub ahead of print - 8 Sept 2019

Bibliographical note

© 2019 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license.

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