Flow cytometric methodology for the detection of de novo human T-cell leukemia virus -1 infection in vitro: a tool to study novel infection inhibitors

Carina Peres; Yuetsu Tanaka; Fabiola Martin; James Martin Fox

Flow cytometric methodology for the detection of de novo human T-cell leukemia virus -1 infection in vitro: a tool to study novel infection inhibitors

Carina Peres, Yuetsu Tanaka, Fabiola Martin, James Martin Fox

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Methodology to detect and study de novo human T-cell leukemia virus (HTLV)-1 infection is
required to further our knowledge of the viruses’ mechanisms of infection and to study
potential therapeutic interventions. Whilst methodology currently exists, utilisation of an anti-
Tax antibody to detect de novo Tax expression in permissive cells labelled with cell tracker
allowing for the detection by flow cytometry of new infection after co-culture with donor cell
lines productively infected with HTLV-1 is an alternative strategy. Using this methodology,
we have been able to detect de novo infection of the T cell line HUT78 following co-culture
with the productively infected HTLV-1 donor cell line MT-2 and to confirm that infection
can be effectively blocked with well characterised infection inhibitors. This methodology will
benefit experimental studies examining HTLV infection in vitro and may aid identification of
therapeutic agents that block this process.

Original language	English
Article number	113728
Journal	Journal of virological methods
Volume	274
Early online date	8 Sep 2019
Publication status	E-pub ahead of print - 8 Sep 2019

Bibliographical note

Access to Document

1-s2.0-S0166093419301934-main
© 2019 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license.
Final published version, 906 KBLicence: CC BY

Cite this

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title = "Flow cytometric methodology for the detection of de novo human T-cell leukemia virus -1 infection in vitro: a tool to study novel infection inhibitors",

abstract = "Methodology to detect and study de novo human T-cell leukemia virus (HTLV)-1 infection isrequired to further our knowledge of the viruses{\textquoteright} mechanisms of infection and to studypotential therapeutic interventions. Whilst methodology currently exists, utilisation of an anti-Tax antibody to detect de novo Tax expression in permissive cells labelled with cell trackerallowing for the detection by flow cytometry of new infection after co-culture with donor celllines productively infected with HTLV-1 is an alternative strategy. Using this methodology,we have been able to detect de novo infection of the T cell line HUT78 following co-culturewith the productively infected HTLV-1 donor cell line MT-2 and to confirm that infectioncan be effectively blocked with well characterised infection inhibitors. This methodology willbenefit experimental studies examining HTLV infection in vitro and may aid identification oftherapeutic agents that block this process.",

author = "Carina Peres and Yuetsu Tanaka and Fabiola Martin and Fox, {James Martin}",

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AU - Peres, Carina

AU - Tanaka, Yuetsu

AU - Martin, Fabiola

AU - Fox, James Martin

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AB - Methodology to detect and study de novo human T-cell leukemia virus (HTLV)-1 infection isrequired to further our knowledge of the viruses’ mechanisms of infection and to studypotential therapeutic interventions. Whilst methodology currently exists, utilisation of an anti-Tax antibody to detect de novo Tax expression in permissive cells labelled with cell trackerallowing for the detection by flow cytometry of new infection after co-culture with donor celllines productively infected with HTLV-1 is an alternative strategy. Using this methodology,we have been able to detect de novo infection of the T cell line HUT78 following co-culturewith the productively infected HTLV-1 donor cell line MT-2 and to confirm that infectioncan be effectively blocked with well characterised infection inhibitors. This methodology willbenefit experimental studies examining HTLV infection in vitro and may aid identification oftherapeutic agents that block this process.

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