Fluorescence quenched quinone methide based activity probes-a cautionary tale.

TY - JOUR

T1 - Fluorescence quenched quinone methide based activity probes-a cautionary tale.

AU - Edwards, Robert

AU - Sellars, J.D

AU - Landrum, M.

AU - Congreve, A.

AU - Dixon, D.P

AU - Moseley, J.A

AU - Beeby, A.

AU - Steel, P.G

PY - 2010

Y1 - 2010

N2 - A carbamate linked quenching group coupled with a pro-quinone methide reactive core provides an effective tool for studying enzyme function without problems associated with background fluorescence from unreacted probe. However, the relatively slow fragmentation of the carbamate linkage in such a strategy may cause problems of loss of signal or a decoupling of enzyme activity and labelling

AB - A carbamate linked quenching group coupled with a pro-quinone methide reactive core provides an effective tool for studying enzyme function without problems associated with background fluorescence from unreacted probe. However, the relatively slow fragmentation of the carbamate linkage in such a strategy may cause problems of loss of signal or a decoupling of enzyme activity and labelling

UR - http://www.scopus.com/inward/record.url?scp=77949795883&partnerID=8YFLogxK

M3 - Article

SN - 1477-0520

VL - 8

SP - 1610

EP - 1618

JO - Organic and Biomolecular Chemistry

JF - Organic and Biomolecular Chemistry

IS - 7

ER -