Fluorochrome-labeled inhibitors of caspase-1 require membrane permeabilization to efficiently access caspase-1 in macrophages

Sara J Thygesen; Sabrina S Burgener; Prerna Mudai; Mercedes Monteleone; Dave Boucher; Vitaliya Sagulenko; Kate Schroder; Katryn J Stacey

doi:10.1002/eji.202350515

Fluorochrome-labeled inhibitors of caspase-1 require membrane permeabilization to efficiently access caspase-1 in macrophages

Sara J Thygesen, Sabrina S Burgener, Prerna Mudai, Mercedes Monteleone, Dave Boucher, Vitaliya Sagulenko, Kate Schroder, Katryn J Stacey

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Caspase-1 location in cells has been studied with fluorochrome-labeled inhibitors of caspase-1 (FLICA reagents). We report that FLICA reagents have limited cell-membrane permeability. This impacts experimental design as cells with intact membranes, including caspase-1 knockout cells, are not appropriate controls for cells with inflammasome-induced gasdermin D membrane pores.

Original language	English
Article number	e2350515
Journal	European Journal of Immunology
Volume	54
Issue number	5
Early online date	15 Feb 2024
DOIs	https://doi.org/10.1002/eji.202350515
Publication status	Published - 7 May 2024

Bibliographical note

Keywords

Caspase 1/metabolism
Animals
Macrophages/immunology
Cell Membrane Permeability/drug effects
Mice
Fluorescent Dyes
Inflammasomes/metabolism
Caspase Inhibitors/pharmacology
Mice, Knockout
Phosphate-Binding Proteins/metabolism
Humans

Access to Document

10.1002/eji.202350515Licence: CC BY

Cite this

@article{6cb1c57e2c4d483ea7a7444e791ebf90,

title = "Fluorochrome-labeled inhibitors of caspase-1 require membrane permeabilization to efficiently access caspase-1 in macrophages",

abstract = "Caspase-1 location in cells has been studied with fluorochrome-labeled inhibitors of caspase-1 (FLICA reagents). We report that FLICA reagents have limited cell-membrane permeability. This impacts experimental design as cells with intact membranes, including caspase-1 knockout cells, are not appropriate controls for cells with inflammasome-induced gasdermin D membrane pores.",

keywords = "Caspase 1/metabolism, Animals, Macrophages/immunology, Cell Membrane Permeability/drug effects, Mice, Fluorescent Dyes, Inflammasomes/metabolism, Caspase Inhibitors/pharmacology, Mice, Knockout, Phosphate-Binding Proteins/metabolism, Humans",

author = "Thygesen, {Sara J} and Burgener, {Sabrina S} and Prerna Mudai and Mercedes Monteleone and Dave Boucher and Vitaliya Sagulenko and Kate Schroder and Stacey, {Katryn J}",

note = "{\textcopyright} 2024 The Authors. European Journal of Immunology published by Wiley‐VCH GmbH.",

year = "2024",

month = may,

day = "7",

doi = "10.1002/eji.202350515",

language = "English",

volume = "54",

journal = "European Journal of Immunology",

issn = "0014-2980",

publisher = "Wiley-Blackwell",

number = "5",

}

TY - JOUR

T1 - Fluorochrome-labeled inhibitors of caspase-1 require membrane permeabilization to efficiently access caspase-1 in macrophages

AU - Thygesen, Sara J

AU - Burgener, Sabrina S

AU - Mudai, Prerna

AU - Monteleone, Mercedes

AU - Boucher, Dave

AU - Sagulenko, Vitaliya

AU - Schroder, Kate

AU - Stacey, Katryn J

PY - 2024/5/7

Y1 - 2024/5/7

N2 - Caspase-1 location in cells has been studied with fluorochrome-labeled inhibitors of caspase-1 (FLICA reagents). We report that FLICA reagents have limited cell-membrane permeability. This impacts experimental design as cells with intact membranes, including caspase-1 knockout cells, are not appropriate controls for cells with inflammasome-induced gasdermin D membrane pores.

AB - Caspase-1 location in cells has been studied with fluorochrome-labeled inhibitors of caspase-1 (FLICA reagents). We report that FLICA reagents have limited cell-membrane permeability. This impacts experimental design as cells with intact membranes, including caspase-1 knockout cells, are not appropriate controls for cells with inflammasome-induced gasdermin D membrane pores.

KW - Caspase 1/metabolism

KW - Animals

KW - Macrophages/immunology

KW - Cell Membrane Permeability/drug effects

KW - Mice

KW - Fluorescent Dyes

KW - Inflammasomes/metabolism

KW - Caspase Inhibitors/pharmacology

KW - Mice, Knockout

KW - Phosphate-Binding Proteins/metabolism

KW - Humans

U2 - 10.1002/eji.202350515

DO - 10.1002/eji.202350515

M3 - Article

C2 - 38361219

SN - 0014-2980

VL - 54

JO - European Journal of Immunology

JF - European Journal of Immunology

IS - 5

M1 - e2350515

ER -