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From EST sequence to spider silk spinning: identification and molecular characterisation of Nephila senegalensis major ampullate gland peroxidase NsPox

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From EST sequence to spider silk spinning: identification and molecular characterisation of Nephila senegalensis major ampullate gland peroxidase NsPox. / Pouchkina, N N; Stanchev, B S; McQueen-Mason, S J.

In: Insect Biochemistry and Molecular Biology, Vol. 33, No. 2, 02.2003, p. 229-238.

Research output: Contribution to journalArticle

Harvard

Pouchkina, NN, Stanchev, BS & McQueen-Mason, SJ 2003, 'From EST sequence to spider silk spinning: identification and molecular characterisation of Nephila senegalensis major ampullate gland peroxidase NsPox', Insect Biochemistry and Molecular Biology, vol. 33, no. 2, pp. 229-238. https://doi.org/10.1016/S0965-1748(02)000207-2

APA

Pouchkina, N. N., Stanchev, B. S., & McQueen-Mason, S. J. (2003). From EST sequence to spider silk spinning: identification and molecular characterisation of Nephila senegalensis major ampullate gland peroxidase NsPox. Insect Biochemistry and Molecular Biology, 33(2), 229-238. https://doi.org/10.1016/S0965-1748(02)000207-2

Vancouver

Pouchkina NN, Stanchev BS, McQueen-Mason SJ. From EST sequence to spider silk spinning: identification and molecular characterisation of Nephila senegalensis major ampullate gland peroxidase NsPox. Insect Biochemistry and Molecular Biology. 2003 Feb;33(2):229-238. https://doi.org/10.1016/S0965-1748(02)000207-2

Author

Pouchkina, N N ; Stanchev, B S ; McQueen-Mason, S J. / From EST sequence to spider silk spinning: identification and molecular characterisation of Nephila senegalensis major ampullate gland peroxidase NsPox. In: Insect Biochemistry and Molecular Biology. 2003 ; Vol. 33, No. 2. pp. 229-238.

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@article{3b5656a6e06d4c95a51f7e1bc618e845,
title = "From EST sequence to spider silk spinning: identification and molecular characterisation of Nephila senegalensis major ampullate gland peroxidase NsPox",
abstract = "Spider dragline silk is renowned as one of the toughest materials of its kind. In nature, spider silks are spun out of aqueous solutions under environmental conditions. This is in contrast to production of most synthetic fibres, where hazardous solvents, high temperatures and pressure are used. In order to identify some of the chemical processes involved in spider silk spinning, we have produced a collection of cDNA sequences from specific regions of Nephila senegalensis major ampullate gland. We examined in detail the sequence and expression of a putative Nephila senegalensis peroxidase gene (NsPox) from our EST collection. NsPox encodes a protein with similarity to Drosophila melanogaster and Aedes aegypti peroxidases. Northern analysis and in situ localisation experiments revealed that NsPox is expressed in major and minor ampullate glands of the spider where the main components of the dragline silk are produced. We suggest that NsPox plays a role in dragline silk fibre formation and/or processing. (C) 2002 Elsevier Science Ltd. All rights reserved.",
keywords = "Nephila senegalensis, spider, dragline silk, silk spinning, peroxidase, MOSQUITO ANOPHELES-ALBIMANUS, AEDES-AEGYPTI, DRAGLINE SILK, MYELOPEROXIDASE, FERTILIZATION, CHORION, PURIFICATION, EVOLUTION, PATHWAY, OXIDASE",
author = "Pouchkina, {N N} and Stanchev, {B S} and McQueen-Mason, {S J}",
year = "2003",
month = "2",
doi = "10.1016/S0965-1748(02)000207-2",
language = "English",
volume = "33",
pages = "229--238",
journal = "Insect Biochemistry and Molecular Biology",
issn = "0965-1748",
publisher = "Elsevier Limited",
number = "2",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - From EST sequence to spider silk spinning: identification and molecular characterisation of Nephila senegalensis major ampullate gland peroxidase NsPox

AU - Pouchkina, N N

AU - Stanchev, B S

AU - McQueen-Mason, S J

PY - 2003/2

Y1 - 2003/2

N2 - Spider dragline silk is renowned as one of the toughest materials of its kind. In nature, spider silks are spun out of aqueous solutions under environmental conditions. This is in contrast to production of most synthetic fibres, where hazardous solvents, high temperatures and pressure are used. In order to identify some of the chemical processes involved in spider silk spinning, we have produced a collection of cDNA sequences from specific regions of Nephila senegalensis major ampullate gland. We examined in detail the sequence and expression of a putative Nephila senegalensis peroxidase gene (NsPox) from our EST collection. NsPox encodes a protein with similarity to Drosophila melanogaster and Aedes aegypti peroxidases. Northern analysis and in situ localisation experiments revealed that NsPox is expressed in major and minor ampullate glands of the spider where the main components of the dragline silk are produced. We suggest that NsPox plays a role in dragline silk fibre formation and/or processing. (C) 2002 Elsevier Science Ltd. All rights reserved.

AB - Spider dragline silk is renowned as one of the toughest materials of its kind. In nature, spider silks are spun out of aqueous solutions under environmental conditions. This is in contrast to production of most synthetic fibres, where hazardous solvents, high temperatures and pressure are used. In order to identify some of the chemical processes involved in spider silk spinning, we have produced a collection of cDNA sequences from specific regions of Nephila senegalensis major ampullate gland. We examined in detail the sequence and expression of a putative Nephila senegalensis peroxidase gene (NsPox) from our EST collection. NsPox encodes a protein with similarity to Drosophila melanogaster and Aedes aegypti peroxidases. Northern analysis and in situ localisation experiments revealed that NsPox is expressed in major and minor ampullate glands of the spider where the main components of the dragline silk are produced. We suggest that NsPox plays a role in dragline silk fibre formation and/or processing. (C) 2002 Elsevier Science Ltd. All rights reserved.

KW - Nephila senegalensis

KW - spider

KW - dragline silk

KW - silk spinning

KW - peroxidase

KW - MOSQUITO ANOPHELES-ALBIMANUS

KW - AEDES-AEGYPTI

KW - DRAGLINE SILK

KW - MYELOPEROXIDASE

KW - FERTILIZATION

KW - CHORION

KW - PURIFICATION

KW - EVOLUTION

KW - PATHWAY

KW - OXIDASE

U2 - 10.1016/S0965-1748(02)000207-2

DO - 10.1016/S0965-1748(02)000207-2

M3 - Article

VL - 33

SP - 229

EP - 238

JO - Insect Biochemistry and Molecular Biology

JF - Insect Biochemistry and Molecular Biology

SN - 0965-1748

IS - 2

ER -