Abstract
Fusicoccin (FC) is a fungal toxin that activates the plant plasma membrane HC-ATPase by binding with 14-3-3 proteins, causing membrane hyperpolarization. Here we report on the effect of FC on a gene-for-gene pathogen-resistance response and show that FC application induces the expression of several genes involved in plant responses to pathogens. Ten members of the PC-binding 14-3-3 protein gene family were isolated from tomato (Lycopersicon esculentum) to characterize their role in defense responses. Sequence analysis is suggestive of common biochemical functions for these tomato 14-3-3 proteins, but their genes showed different expression patterns in leaves after challenges. Different specific subsets of 14-3-3 genes were induced after treatment with FC and during a gene-for-gene resistance response. Possible roles for the H+-ATPase and 14-3-3 proteins in responses to pathogens are discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 1243-1250 |
| Number of pages | 8 |
| Journal | Plant Physiology |
| Volume | 119 |
| Issue number | 4 |
| Publication status | Published - Apr 1999 |
Keywords
- MEMBRANE H+-ATPASE
- MULTIPLE SIGNALING PATHWAYS
- ISOFORM-SPECIFIC MANNER
- BINDING COMPLEX
- 14-3-3-PROTEINS ASSOCIATE
- ARABIDOPSIS-THALIANA
- REGULATORY PROTEINS
- NITRATE REDUCTASE
- FUNGAL PATHOGENS
- PROTON PUMP