General in vitro caspase assay procedures

Dave Boucher; Catherine Duclos; Jean-Bernard Denault

doi:10.1007/978-1-4939-0357-3_1

General in vitro caspase assay procedures

Dave Boucher, Catherine Duclos, Jean-Bernard Denault

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

One of the most valuable tools that have been developed for the study of apoptosis is the availability of recombinant active caspases. The determination of caspase substrate preference, the design of sensitive substrates and potent inhibitors, the resolution of caspase structures, the elucidation of their activation mechanisms, and the identification of their substrates were made possible by the availability of sufficient amounts of enzymatically pure caspases. The current chapter describes at length the expression, purification, and basic enzymatic characterization of apoptotic caspases.

Original language	English
Pages (from-to)	3-39
Number of pages	37
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	1133
Early online date	8 Feb 2014
DOIs	https://doi.org/10.1007/978-1-4939-0357-3_1
Publication status	Published - 2014

Keywords

Apoptosis/genetics
Caspase Inhibitors/chemistry
Caspases/chemistry
Enzyme Activation
Humans
Molecular Biology/methods
Recombinant Proteins/chemistry
Substrate Specificity

Access to Document

10.1007/978-1-4939-0357-3_1

Cite this

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AU - Denault, Jean-Bernard

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KW - Apoptosis/genetics

KW - Caspase Inhibitors/chemistry

KW - Caspases/chemistry

KW - Enzyme Activation

KW - Humans

KW - Molecular Biology/methods

KW - Recombinant Proteins/chemistry

KW - Substrate Specificity

U2 - 10.1007/978-1-4939-0357-3_1

DO - 10.1007/978-1-4939-0357-3_1

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JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

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