Genetic diversity assessment in winter oilseed rape (Brassica napus L.) collection using AFLP, ISSR and SSR markers

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Genetic diversity assessment in winter oilseed rape (Brassica napus L.) collection using AFLP, ISSR and SSR markers. / Havlíčková, Lenka; Jozová, Eva; Rychlá, Andrea; Klíma, Miroslav; Kučera, Vratislav; Čurn, Vladislav.

In: Czech Journal of Genetics and Plant Breeding, Vol. 50, No. 3, 2014, p. 216-225.

Research output: Contribution to journalArticle

Harvard

Havlíčková, L, Jozová, E, Rychlá, A, Klíma, M, Kučera, V & Čurn, V 2014, 'Genetic diversity assessment in winter oilseed rape (Brassica napus L.) collection using AFLP, ISSR and SSR markers', Czech Journal of Genetics and Plant Breeding, vol. 50, no. 3, pp. 216-225.

APA

Havlíčková, L., Jozová, E., Rychlá, A., Klíma, M., Kučera, V., & Čurn, V. (2014). Genetic diversity assessment in winter oilseed rape (Brassica napus L.) collection using AFLP, ISSR and SSR markers. Czech Journal of Genetics and Plant Breeding, 50(3), 216-225.

Vancouver

Havlíčková L, Jozová E, Rychlá A, Klíma M, Kučera V, Čurn V. Genetic diversity assessment in winter oilseed rape (Brassica napus L.) collection using AFLP, ISSR and SSR markers. Czech Journal of Genetics and Plant Breeding. 2014;50(3):216-225.

Author

Havlíčková, Lenka ; Jozová, Eva ; Rychlá, Andrea ; Klíma, Miroslav ; Kučera, Vratislav ; Čurn, Vladislav. / Genetic diversity assessment in winter oilseed rape (Brassica napus L.) collection using AFLP, ISSR and SSR markers. In: Czech Journal of Genetics and Plant Breeding. 2014 ; Vol. 50, No. 3. pp. 216-225.

Bibtex - Download

@article{715c1661f6654005bf38533b3d8e08f8,
title = "Genetic diversity assessment in winter oilseed rape (Brassica napus L.) collection using AFLP, ISSR and SSR markers",
abstract = "The genetic diversity of 94 accessions of winter oilseed rape (Brassica napus L.), representing past and contemporary material utilized in the Czech breeding programmes, was determined using microsatellites (SSRs), ISSRs and AFLPs. All three kinds of markers differed in the range of observed polymorphism and differentiated clearly each accession. Altogether 89 SSR, 1003 AFLP and 53 ISSR markers were evaluated. Their average rates of polymorphic bands were 100{\%}, 53.9{\%} and 90.6{\%}, respectively, indicating high genetic diversity among the accessions. The greatest genetic distance was found by ISSRs (62.3{\%}) whereas significantly lower distances of 49.4{\%} in SSRs and 35.5{\%} in AFLPs were observed. The genetic similarity matrix clearly distinguished all accessions. A set of the most distinct varieties was established. The analysis of the genetic pattern of the accessions indicated two groups comprising most of the modern Czech breeding materials, revealing a distinct shift in breeding. Surprisingly, molecular analyses did not support breeders’ views about a narrow genetic base of the Czech breeding materials. The choice of appropriate technology for different aspects of germplasm evaluation is also discussed.",
keywords = "Brassica napus, Genetic distances, Genetic diversity, Molecular markers",
author = "Lenka Havl{\'i}čkov{\'a} and Eva Jozov{\'a} and Andrea Rychl{\'a} and Miroslav Kl{\'i}ma and Vratislav Kučera and Vladislav Čurn",
year = "2014",
language = "English",
volume = "50",
pages = "216--225",
journal = "Czech Journal of Genetics and Plant Breeding",
issn = "1212-1975",
publisher = "Czech Academy of Agricultural Sciences",
number = "3",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Genetic diversity assessment in winter oilseed rape (Brassica napus L.) collection using AFLP, ISSR and SSR markers

AU - Havlíčková, Lenka

AU - Jozová, Eva

AU - Rychlá, Andrea

AU - Klíma, Miroslav

AU - Kučera, Vratislav

AU - Čurn, Vladislav

PY - 2014

Y1 - 2014

N2 - The genetic diversity of 94 accessions of winter oilseed rape (Brassica napus L.), representing past and contemporary material utilized in the Czech breeding programmes, was determined using microsatellites (SSRs), ISSRs and AFLPs. All three kinds of markers differed in the range of observed polymorphism and differentiated clearly each accession. Altogether 89 SSR, 1003 AFLP and 53 ISSR markers were evaluated. Their average rates of polymorphic bands were 100%, 53.9% and 90.6%, respectively, indicating high genetic diversity among the accessions. The greatest genetic distance was found by ISSRs (62.3%) whereas significantly lower distances of 49.4% in SSRs and 35.5% in AFLPs were observed. The genetic similarity matrix clearly distinguished all accessions. A set of the most distinct varieties was established. The analysis of the genetic pattern of the accessions indicated two groups comprising most of the modern Czech breeding materials, revealing a distinct shift in breeding. Surprisingly, molecular analyses did not support breeders’ views about a narrow genetic base of the Czech breeding materials. The choice of appropriate technology for different aspects of germplasm evaluation is also discussed.

AB - The genetic diversity of 94 accessions of winter oilseed rape (Brassica napus L.), representing past and contemporary material utilized in the Czech breeding programmes, was determined using microsatellites (SSRs), ISSRs and AFLPs. All three kinds of markers differed in the range of observed polymorphism and differentiated clearly each accession. Altogether 89 SSR, 1003 AFLP and 53 ISSR markers were evaluated. Their average rates of polymorphic bands were 100%, 53.9% and 90.6%, respectively, indicating high genetic diversity among the accessions. The greatest genetic distance was found by ISSRs (62.3%) whereas significantly lower distances of 49.4% in SSRs and 35.5% in AFLPs were observed. The genetic similarity matrix clearly distinguished all accessions. A set of the most distinct varieties was established. The analysis of the genetic pattern of the accessions indicated two groups comprising most of the modern Czech breeding materials, revealing a distinct shift in breeding. Surprisingly, molecular analyses did not support breeders’ views about a narrow genetic base of the Czech breeding materials. The choice of appropriate technology for different aspects of germplasm evaluation is also discussed.

KW - Brassica napus

KW - Genetic distances

KW - Genetic diversity

KW - Molecular markers

UR - http://www.scopus.com/inward/record.url?scp=84907143438&partnerID=8YFLogxK

M3 - Article

VL - 50

SP - 216

EP - 225

JO - Czech Journal of Genetics and Plant Breeding

T2 - Czech Journal of Genetics and Plant Breeding

JF - Czech Journal of Genetics and Plant Breeding

SN - 1212-1975

IS - 3

ER -