TY - JOUR
T1 - Genetic diversity of eight millet genera assessed by using molecular and morphological markers
AU - Dvořáková, Zdislava
AU - Čepková, Petra Hlásná
AU - Viehmannová, Iva
AU - Havlicková, Lenka
AU - Janovská, Dagmar
PY - 2016/2/29
Y1 - 2016/2/29
N2 - In this study, the genetic diversity and relationships among eight millet genera were investigated by molecular and morphological data analyses. Sixty-nine millet accessions were analysed by using amplified fragment length polymorphism (AFLP) markers, and evaluated for morphological traits. Eight AFLP primer pairs were amplified successfully and 779 bands were scored for all accessions, with a high level of polymorphism detected. Nei's genetic distance among all accessions varied from 0.0123 to 0.4246 and the Shannon's index was estimated at 0.9708. The neighbour joining tree, using the unweighted neighbour-joining method and Dice's dissimilarity coefficient, was constructed. The AFLP markers revealed the close relatedness between the Eragrostis and Panicum genera, whereas the greatest distance was found the Pennisetum and Echinochloa genera. Cluster analysis based on the AFLP profiles revealed that the majority of accessions of a given millet genus tend to group together. Clustering from morphological data allocated individuals into three main clusters with high variation. The genetic variability found between the analysed accessions was weakly negatively correlated (r = -0.074) with their morphological attributes. However, high molecular and morphological variability indicated that this collection includes rich and valuable plant materials for millet breeding.
AB - In this study, the genetic diversity and relationships among eight millet genera were investigated by molecular and morphological data analyses. Sixty-nine millet accessions were analysed by using amplified fragment length polymorphism (AFLP) markers, and evaluated for morphological traits. Eight AFLP primer pairs were amplified successfully and 779 bands were scored for all accessions, with a high level of polymorphism detected. Nei's genetic distance among all accessions varied from 0.0123 to 0.4246 and the Shannon's index was estimated at 0.9708. The neighbour joining tree, using the unweighted neighbour-joining method and Dice's dissimilarity coefficient, was constructed. The AFLP markers revealed the close relatedness between the Eragrostis and Panicum genera, whereas the greatest distance was found the Pennisetum and Echinochloa genera. Cluster analysis based on the AFLP profiles revealed that the majority of accessions of a given millet genus tend to group together. Clustering from morphological data allocated individuals into three main clusters with high variation. The genetic variability found between the analysed accessions was weakly negatively correlated (r = -0.074) with their morphological attributes. However, high molecular and morphological variability indicated that this collection includes rich and valuable plant materials for millet breeding.
KW - Millet species
KW - Morphological traits
KW - Relatedness
UR - http://www.scopus.com/inward/record.url?scp=84985896497&partnerID=8YFLogxK
U2 - 10.1071/CP15202
DO - 10.1071/CP15202
M3 - Article
AN - SCOPUS:84985896497
SN - 1836-0947
VL - 67
SP - 181
EP - 192
JO - Crop and Pasture Science
JF - Crop and Pasture Science
IS - 2
ER -