@inbook{0635af8218a64ef1b54bf349245851ae,
title = "Genetic Techniques for Manipulation of the Phytosterol Biotransformation Strain Mycobacterium neoaurum NRRL B-3805",
abstract = "Mycobacterium neoaurum is a saprophytic, soil-dwelling bacterium. The strain NRRL B-3805 converts phytosterols to androst-4-ene-3,17-dione (androstenedione; AD), a precursor of multiple C19 steroids of importance to industry. NRRL B-3805 itself is able to convert AD to other steroid products, including testosterone (Ts) and androst-1,4-diene-3,17-dione (androstadienedione; ADD). However to improve this strain for industrial use, genetic modification is a priority. In this chapter, we describe a range of genetic techniques that can be used for M. neoaurum NRRL B-3805. Methods for transformation, expression, and gene knockouts are presented as well as plasmid maintenance and stability.",
keywords = "Journal Article",
author = "Loraine, {Jessica K} and Smith, {Margaret C M}",
year = "2017",
doi = "10.1007/978-1-4939-7183-1_7",
language = "English",
isbn = "978-1-4939-7182-4",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "93--108",
editor = "Barredo, {Jos{\'e}-Luis } and Ignacio Herr{\'a}iz",
booktitle = "Microbial Steroids",
}