Genomic analysis of a lignocellulose degrading strain from the underexplored genus Meridianimaribacter

TY - JOUR

T1 - Genomic analysis of a lignocellulose degrading strain from the underexplored genus Meridianimaribacter

AU - Quan Lam, Ming

AU - Oates, Nicola Claire

AU - Thevarajoo, Suganthi

AU - Tokiman, Lili

AU - Mau Goh, Kian

AU - McQueen Mason, Simon John

AU - Bruce, Neil Charles

AU - Chong, Chun Shiong

N1 - © 2019 Elsevier Inc. All rights reserved. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy.

PY - 2019/6/13

Y1 - 2019/6/13

N2 - The genus Meridianimaribacter is one of the least-studied genera within Cytophaga-Flavobacteria. To date, no genomic analysis of Meridianimaribacter has beenreported. In this study, Meridianimaribacter sp. strain CL38, a lignocellulosedegrading halophile was isolated from mangrove soil. The genome of strain CL38was sequenced and analyzed. The assembled genome contains 17 contigs with 3.33Mbp, a GC content of 33.13% and a total of 2982 genes predicted. Lignocellulosedegrading enzymes such as cellulases (GH3, 5, 9, 16, 74 and 144), xylanases (GH43and CE4) and mannanases (GH5, 26, 27 and 130) are encoded in the genome.Furthermore, strain CL38 demonstrated its ability to decompose empty fruit bunch, alignocellulosic waste residue arising from palm oil industry. The genome informationcoupled with experimental studies confirmed the ability of strain CL38 to degradelignocellulosic biomass. Therefore, Meridianimaribacter sp. strain CL38, with itshalotolerance, could be useful for seawater based lignocellulosic biorefining.

AB - The genus Meridianimaribacter is one of the least-studied genera within Cytophaga-Flavobacteria. To date, no genomic analysis of Meridianimaribacter has beenreported. In this study, Meridianimaribacter sp. strain CL38, a lignocellulosedegrading halophile was isolated from mangrove soil. The genome of strain CL38was sequenced and analyzed. The assembled genome contains 17 contigs with 3.33Mbp, a GC content of 33.13% and a total of 2982 genes predicted. Lignocellulosedegrading enzymes such as cellulases (GH3, 5, 9, 16, 74 and 144), xylanases (GH43and CE4) and mannanases (GH5, 26, 27 and 130) are encoded in the genome.Furthermore, strain CL38 demonstrated its ability to decompose empty fruit bunch, alignocellulosic waste residue arising from palm oil industry. The genome informationcoupled with experimental studies confirmed the ability of strain CL38 to degradelignocellulosic biomass. Therefore, Meridianimaribacter sp. strain CL38, with itshalotolerance, could be useful for seawater based lignocellulosic biorefining.

KW - Empty fruit bunch

KW - Genomics

KW - Halophile

KW - Lignocellulolytic enzymes

KW - Meridianimaribacter

UR - http://www.scopus.com/inward/record.url?scp=85067271740&partnerID=8YFLogxK

U2 - 10.1016/j.ygeno.2019.06.011

DO - 10.1016/j.ygeno.2019.06.011

M3 - Article

SN - 0888-7543

JO - Genomics

JF - Genomics

ER -