By the same authors

From the same journal

From the same journal

Genomic RNA folding mediates assembly of human parechovirus

Research output: Contribution to journalArticle

Full text download(s)

Published copy (DOI)

Author(s)

Department/unit(s)

Publication details

JournalNature Communications
DateAccepted/In press - 16 Nov 2016
DateE-pub ahead of print (current) - 23 Feb 2017
Issue number5
Volume8
Number of pages12
Early online date23/02/17
Original languageEnglish

Abstract

Assembly of the major viral pathogens of the Picornaviridae family is poorly understood. Human parechovirus 1 is an example of such viruses that contains 60 short regions of ordered RNA density making identical contacts with the protein shell. We show here via a combination of RNA-based systematic evolution of ligands by exponential enrichment, bioinformatics analysis and reverse genetics that these RNA segments are bound to the coat proteins in a sequence-specific manner. Disruption of either the RNA coat protein recognition motif or its contact amino acid residues is deleterious for viral assembly. The data are consistent with RNA packaging signals playing essential roles in virion assembly. Their binding sites on the coat proteins are evolutionarily conserved across the Parechovirus genus, suggesting that they represent potential broad-spectrum anti-viral targets.

Bibliographical note

© 2017, The Authors.

Discover related content

Find related publications, people, projects, datasets and more using interactive charts.

View graph of relations