Genotype, phenotype, and protein structure in a regulator of sporulation: Effects of mutations in the spoIIAA gene of Bacillus subtilis

D  Barilla; I  Lucet; A  Kuhlmann; M D  Yudkin

Genotype, phenotype, and protein structure in a regulator of sporulation: Effects of mutations in the spoIIAA gene of Bacillus subtilis

D Barilla, I Lucet, A Kuhlmann, M D Yudkin

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

SpoIIAA, a phosphorylatable protein, is essential to the regulation of sigma(F), the first sporulation-specific transcription factor of Bacillus subtilis. The solution structure of SpoIIAA has recently been published. Here we examine four mutant SpoIIAA proteins and correlate their properties with the phenotypes of the corresponding B. subtilis mutant strains. Two of the mutations severely disrupted the structure of the protein, a third greatly diminished the rate of its phosphorylation and abolished dephosphorylation, and the fourth left phosphorylation unaffected but reduced the rate of dephosphorylation about 10-fold.

Original language	English
Pages (from-to)	3860-3863
Number of pages	4
Journal	Journal of Bacteriology
Volume	181
Issue number	12
Publication status	Published - Jun 1999

Keywords

TRANSCRIPTION FACTOR SIGMA(F)
CELL-SPECIFIC TRANSCRIPTION
EXPRESSION
DIVISION
SITE

Cite this

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title = "Genotype, phenotype, and protein structure in a regulator of sporulation: Effects of mutations in the spoIIAA gene of Bacillus subtilis",

abstract = "SpoIIAA, a phosphorylatable protein, is essential to the regulation of sigma(F), the first sporulation-specific transcription factor of Bacillus subtilis. The solution structure of SpoIIAA has recently been published. Here we examine four mutant SpoIIAA proteins and correlate their properties with the phenotypes of the corresponding B. subtilis mutant strains. Two of the mutations severely disrupted the structure of the protein, a third greatly diminished the rate of its phosphorylation and abolished dephosphorylation, and the fourth left phosphorylation unaffected but reduced the rate of dephosphorylation about 10-fold.",

keywords = "TRANSCRIPTION FACTOR SIGMA(F), CELL-SPECIFIC TRANSCRIPTION, EXPRESSION, DIVISION, SITE",

author = "D Barilla and I Lucet and A Kuhlmann and Yudkin, {M D}",

year = "1999",

month = jun,

language = "English",

volume = "181",

pages = "3860--3863",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

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TY - JOUR

T1 - Genotype, phenotype, and protein structure in a regulator of sporulation: Effects of mutations in the spoIIAA gene of Bacillus subtilis

AU - Barilla, D

AU - Lucet, I

AU - Kuhlmann, A

AU - Yudkin, M D

PY - 1999/6

Y1 - 1999/6

N2 - SpoIIAA, a phosphorylatable protein, is essential to the regulation of sigma(F), the first sporulation-specific transcription factor of Bacillus subtilis. The solution structure of SpoIIAA has recently been published. Here we examine four mutant SpoIIAA proteins and correlate their properties with the phenotypes of the corresponding B. subtilis mutant strains. Two of the mutations severely disrupted the structure of the protein, a third greatly diminished the rate of its phosphorylation and abolished dephosphorylation, and the fourth left phosphorylation unaffected but reduced the rate of dephosphorylation about 10-fold.

AB - SpoIIAA, a phosphorylatable protein, is essential to the regulation of sigma(F), the first sporulation-specific transcription factor of Bacillus subtilis. The solution structure of SpoIIAA has recently been published. Here we examine four mutant SpoIIAA proteins and correlate their properties with the phenotypes of the corresponding B. subtilis mutant strains. Two of the mutations severely disrupted the structure of the protein, a third greatly diminished the rate of its phosphorylation and abolished dephosphorylation, and the fourth left phosphorylation unaffected but reduced the rate of dephosphorylation about 10-fold.

KW - TRANSCRIPTION FACTOR SIGMA(F)

KW - CELL-SPECIFIC TRANSCRIPTION

KW - EXPRESSION

KW - DIVISION

KW - SITE

M3 - Article

SN - 0021-9193

VL - 181

SP - 3860

EP - 3863

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 12

ER -