Germ-line transformation of the Mexican fruit fly

K. C. Condon; G. C. Condon; T. H. Dafa'alla; O. T. Forrester; C. E. Phillips; S. Scaife; L. Alphey

doi:10.1111/j.1365-2583.2007.00752.x

Germ-line transformation of the Mexican fruit fly

K. C. Condon, G. C. Condon, T. H. Dafa'alla, O. T. Forrester, C. E. Phillips, S. Scaife, L. Alphey^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Germ-line transformation of a major agricultural pest, the Mexican fruit fly (Anastrepha ludens Loew, Mexfly), was achieved using composite piggyBac transposable elements marked with green, yellow and red fluorescent proteins (CopGreen, PhiYFP and J-Red). We also investigated the possibility of generating transposon-free insertions, in order to address potential concerns relating to proposed field use of transgenic Mexfly. We describe a highly efficient method for transforming Mexfly, compare efficiency of piggyBac terminal sequences for transformation and also describe the derivation of a transposon-free insertion line. The development of an efficient transformation system for Mexfly holds great promise for improved applications of the sterile insect technique, a major component of the present control measures for this economically important pest species.

Original language	English
Pages (from-to)	573-580
Number of pages	8
Journal	Insect Molecular Biology
Volume	16
Issue number	5
DOIs	https://doi.org/10.1111/j.1365-2583.2007.00752.x
Publication status	Published - Oct 2007

Keywords

Anastrepha ludens
Germ-line transformation
Mexican fruit fly
piggyBac
Transposon-free insertion

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10.1111/j.1365-2583.2007.00752.x

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title = "Germ-line transformation of the Mexican fruit fly",

abstract = "Germ-line transformation of a major agricultural pest, the Mexican fruit fly (Anastrepha ludens Loew, Mexfly), was achieved using composite piggyBac transposable elements marked with green, yellow and red fluorescent proteins (CopGreen, PhiYFP and J-Red). We also investigated the possibility of generating transposon-free insertions, in order to address potential concerns relating to proposed field use of transgenic Mexfly. We describe a highly efficient method for transforming Mexfly, compare efficiency of piggyBac terminal sequences for transformation and also describe the derivation of a transposon-free insertion line. The development of an efficient transformation system for Mexfly holds great promise for improved applications of the sterile insect technique, a major component of the present control measures for this economically important pest species.",

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AU - Condon, K. C.

AU - Condon, G. C.

AU - Dafa'alla, T. H.

AU - Forrester, O. T.

AU - Phillips, C. E.

AU - Scaife, S.

AU - Alphey, L.

PY - 2007/10

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AB - Germ-line transformation of a major agricultural pest, the Mexican fruit fly (Anastrepha ludens Loew, Mexfly), was achieved using composite piggyBac transposable elements marked with green, yellow and red fluorescent proteins (CopGreen, PhiYFP and J-Red). We also investigated the possibility of generating transposon-free insertions, in order to address potential concerns relating to proposed field use of transgenic Mexfly. We describe a highly efficient method for transforming Mexfly, compare efficiency of piggyBac terminal sequences for transformation and also describe the derivation of a transposon-free insertion line. The development of an efficient transformation system for Mexfly holds great promise for improved applications of the sterile insect technique, a major component of the present control measures for this economically important pest species.

KW - Anastrepha ludens

KW - Germ-line transformation

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KW - piggyBac

KW - Transposon-free insertion

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Germ-line transformation of the Mexican fruit fly

Abstract

Keywords

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