Germ-Line Transmitted, Chromosomally Integrated HHV-6 and Classical Hodgkin Lymphoma

Adam J Bell; Alice Gallagher; Timothy Mottram; Annette Lake; Eleanor V Kane; Tracy Lightfoot; Eve Roman; Ruth F Jarrett

doi:10.1371/journal.pone.0112642

Germ-Line Transmitted, Chromosomally Integrated HHV-6 and Classical Hodgkin Lymphoma

Adam J Bell
, Alice Gallagher
, Timothy Mottram
, Annette Lake
, Eleanor V Kane
, Tracy Lightfoot
, Eve Roman
, Ruth F Jarrett

Health Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

A unique feature of both human herpesvirus 6A and B (HHV-6A and B) among human herpesviruses is their ability to integrate into chromosomal telomeres. In some individuals integrated viral genomes are present in the germ-line and result in the vertical transmission of HHV-6; however, little is known about the disease associations of germ-line transmitted, chromosomally integrated HHV-6 (ciHHV-6). Recent publications suggest that HHV-6 is associated with classical Hodgkin lymphoma (cHL). Here we examine the prevalence of ciHHV-6 in 936 cases of cHL and 563 controls by screening with a duplex TaqMan assay and confirming with droplet digital PCR. ciHHV-6 was detected in 10/563 (1.8%) controls and in all but one individual the virus was HHV-6B. Amongst cases 16/936 (1.7%) harboured ciHHV-6, thus demonstrating no association between ciHHV-6 and risk of cHL.

Original language	English
Article number	e112642
Number of pages	8
Journal	PLoS ONE
Volume	9
Issue number	11
DOIs	https://doi.org/10.1371/journal.pone.0112642
Publication status	Published - 10 Nov 2014

Access to Document

10.1371/journal.pone.0112642

Cite this

@article{158ac4816c8f4474b4c9ed2f96adc16c,

title = "Germ-Line Transmitted, Chromosomally Integrated HHV-6 and Classical Hodgkin Lymphoma",

abstract = "A unique feature of both human herpesvirus 6A and B (HHV-6A and B) among human herpesviruses is their ability to integrate into chromosomal telomeres. In some individuals integrated viral genomes are present in the germ-line and result in the vertical transmission of HHV-6; however, little is known about the disease associations of germ-line transmitted, chromosomally integrated HHV-6 (ciHHV-6). Recent publications suggest that HHV-6 is associated with classical Hodgkin lymphoma (cHL). Here we examine the prevalence of ciHHV-6 in 936 cases of cHL and 563 controls by screening with a duplex TaqMan assay and confirming with droplet digital PCR. ciHHV-6 was detected in 10/563 (1.8\%) controls and in all but one individual the virus was HHV-6B. Amongst cases 16/936 (1.7\%) harboured ciHHV-6, thus demonstrating no association between ciHHV-6 and risk of cHL.",

author = "Bell, \{Adam J\} and Alice Gallagher and Timothy Mottram and Annette Lake and Kane, \{Eleanor V\} and Tracy Lightfoot and Eve Roman and Jarrett, \{Ruth F\}",

year = "2014",

month = nov,

day = "10",

doi = "10.1371/journal.pone.0112642",

language = "English",

volume = "9",

journal = "PLoS ONE",

issn = "1932-6203",

publisher = "Public Library of Science",

number = "11",

}

TY - JOUR

T1 - Germ-Line Transmitted, Chromosomally Integrated HHV-6 and Classical Hodgkin Lymphoma

AU - Bell, Adam J

AU - Gallagher, Alice

AU - Mottram, Timothy

AU - Lake, Annette

AU - Kane, Eleanor V

AU - Lightfoot, Tracy

AU - Roman, Eve

AU - Jarrett, Ruth F

PY - 2014/11/10

Y1 - 2014/11/10

N2 - A unique feature of both human herpesvirus 6A and B (HHV-6A and B) among human herpesviruses is their ability to integrate into chromosomal telomeres. In some individuals integrated viral genomes are present in the germ-line and result in the vertical transmission of HHV-6; however, little is known about the disease associations of germ-line transmitted, chromosomally integrated HHV-6 (ciHHV-6). Recent publications suggest that HHV-6 is associated with classical Hodgkin lymphoma (cHL). Here we examine the prevalence of ciHHV-6 in 936 cases of cHL and 563 controls by screening with a duplex TaqMan assay and confirming with droplet digital PCR. ciHHV-6 was detected in 10/563 (1.8%) controls and in all but one individual the virus was HHV-6B. Amongst cases 16/936 (1.7%) harboured ciHHV-6, thus demonstrating no association between ciHHV-6 and risk of cHL.

AB - A unique feature of both human herpesvirus 6A and B (HHV-6A and B) among human herpesviruses is their ability to integrate into chromosomal telomeres. In some individuals integrated viral genomes are present in the germ-line and result in the vertical transmission of HHV-6; however, little is known about the disease associations of germ-line transmitted, chromosomally integrated HHV-6 (ciHHV-6). Recent publications suggest that HHV-6 is associated with classical Hodgkin lymphoma (cHL). Here we examine the prevalence of ciHHV-6 in 936 cases of cHL and 563 controls by screening with a duplex TaqMan assay and confirming with droplet digital PCR. ciHHV-6 was detected in 10/563 (1.8%) controls and in all but one individual the virus was HHV-6B. Amongst cases 16/936 (1.7%) harboured ciHHV-6, thus demonstrating no association between ciHHV-6 and risk of cHL.

U2 - 10.1371/journal.pone.0112642

DO - 10.1371/journal.pone.0112642

M3 - Article

C2 - 25384040

SN - 1932-6203

VL - 9

JO - PLoS ONE

JF - PLoS ONE

IS - 11

M1 - e112642

ER -