Glycosylated cyclophellitol-derived activity-based probes and inhibitors for cellulases

Casper De Boer, Nicholas George Sarre McGregor, Evert Peterse, Sybrin P Schröder, Bogdan I. Florea, Jianbing Jiang, Jos Reijngoud, Arthur F.J. Ram, Gijsbert A. Van Der Marel, Gilles P. Van Wezel, Jeroen D. C. Codee, Herman S. Overkleeft, Gideon John Davies

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Cellulases and related β-1,4-glucanases are essential components of lignocellulose-degrading enzyme mixtures. The detection of β-1,4-glucanase activity typically relies on monitoring the breakdown of purified lignocellulose-derived substrates or synthetic chromogenic substrates, limiting the activities which can be detected and complicating the tracing of activity back to specific components within complex enzyme mixtures. As a tool for the rapid detection and identification of β-1,4-glucanases, a series of glycosylated cyclophellitol inhibitors mimicking β-1,4-glucan oligosaccharides have been synthesised. These compounds are highly efficient inhibitors of HiCel7B, a well-known GH7 endo -β-1,4-glucanase. An elaborated activity-based probe facilitated the direct detection and identification of β-1,4-glucanases within a complex fungal secretome without any detectable cross-reactivity with β- d -glucosidases. These probes and inhibitors add valuable new capacity to the growing toolbox of cyclophellitol-derived probes for the activity-based profiling of biomass-degrading enzymes.
Original languageEnglish
Pages (from-to)148-155
Number of pages8
JournalRSC Chemical Biology
Issue number3
Publication statusPublished - 28 Jul 2020

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© The Royal Society of Chemistry 2020

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