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Glycosylated cyclophellitol-derived activity-based probes and inhibitors for cellulases

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Glycosylated cyclophellitol-derived activity-based probes and inhibitors for cellulases. / De Boer, Casper; McGregor, Nicholas George Sarre; Peterse, Evert; Schröder, Sybrin P; Florea, Bogdan I.; Jiang, Jianbing; Reijngoud, Jos; Ram, Arthur F.J.; Van Der Marel, Gijsbert A.; Van Wezel, Gilles P.; Codee, Jeroen D. C. ; Overkleeft, Herman S.; Davies, Gideon John.

In: RSC Chemical Biology, Vol. 1, No. 3, 28.07.2020, p. 148-155.

Research output: Contribution to journalArticle

Harvard

De Boer, C, McGregor, NGS, Peterse, E, Schröder, SP, Florea, BI, Jiang, J, Reijngoud, J, Ram, AFJ, Van Der Marel, GA, Van Wezel, GP, Codee, JDC, Overkleeft, HS & Davies, GJ 2020, 'Glycosylated cyclophellitol-derived activity-based probes and inhibitors for cellulases', RSC Chemical Biology, vol. 1, no. 3, pp. 148-155. https://doi.org/10.1039/D0CB00045K

APA

De Boer, C., McGregor, N. G. S., Peterse, E., Schröder, S. P., Florea, B. I., Jiang, J., Reijngoud, J., Ram, A. F. J., Van Der Marel, G. A., Van Wezel, G. P., Codee, J. D. C., Overkleeft, H. S., & Davies, G. J. (2020). Glycosylated cyclophellitol-derived activity-based probes and inhibitors for cellulases. RSC Chemical Biology, 1(3), 148-155. https://doi.org/10.1039/D0CB00045K

Vancouver

De Boer C, McGregor NGS, Peterse E, Schröder SP, Florea BI, Jiang J et al. Glycosylated cyclophellitol-derived activity-based probes and inhibitors for cellulases. RSC Chemical Biology. 2020 Jul 28;1(3):148-155. https://doi.org/10.1039/D0CB00045K

Author

De Boer, Casper ; McGregor, Nicholas George Sarre ; Peterse, Evert ; Schröder, Sybrin P ; Florea, Bogdan I. ; Jiang, Jianbing ; Reijngoud, Jos ; Ram, Arthur F.J. ; Van Der Marel, Gijsbert A. ; Van Wezel, Gilles P. ; Codee, Jeroen D. C. ; Overkleeft, Herman S. ; Davies, Gideon John. / Glycosylated cyclophellitol-derived activity-based probes and inhibitors for cellulases. In: RSC Chemical Biology. 2020 ; Vol. 1, No. 3. pp. 148-155.

Bibtex - Download

@article{823ad2de7a1144d0bcd95e859fa3ece8,
title = "Glycosylated cyclophellitol-derived activity-based probes and inhibitors for cellulases",
abstract = "Cellulases and related β-1,4-glucanases are essential components of lignocellulose-degrading enzyme mixtures. The detection of β-1,4-glucanase activity typically relies on monitoring the breakdown of purified lignocellulose-derived substrates or synthetic chromogenic substrates, limiting the activities which can be detected and complicating the tracing of activity back to specific components within complex enzyme mixtures. As a tool for the rapid detection and identification of β-1,4-glucanases, a series of glycosylated cyclophellitol inhibitors mimicking β-1,4-glucan oligosaccharides have been synthesised. These compounds are highly efficient inhibitors of HiCel7B, a well-known GH7 endo -β-1,4-glucanase. An elaborated activity-based probe facilitated the direct detection and identification of β-1,4-glucanases within a complex fungal secretome without any detectable cross-reactivity with β- d -glucosidases. These probes and inhibitors add valuable new capacity to the growing toolbox of cyclophellitol-derived probes for the activity-based profiling of biomass-degrading enzymes. ",
author = "{De Boer}, Casper and McGregor, {Nicholas George Sarre} and Evert Peterse and Schr{\"o}der, {Sybrin P} and Florea, {Bogdan I.} and Jianbing Jiang and Jos Reijngoud and Ram, {Arthur F.J.} and {Van Der Marel}, {Gijsbert A.} and {Van Wezel}, {Gilles P.} and Codee, {Jeroen D. C.} and Overkleeft, {Herman S.} and Davies, {Gideon John}",
note = "{\textcopyright} The Royal Society of Chemistry 2020",
year = "2020",
month = jul,
day = "28",
doi = "10.1039/D0CB00045K",
language = "English",
volume = "1",
pages = "148--155",
journal = "RSC Chemical Biology",
issn = "2633-0679",
publisher = "Royal Society of Chemistry",
number = "3",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Glycosylated cyclophellitol-derived activity-based probes and inhibitors for cellulases

AU - De Boer, Casper

AU - McGregor, Nicholas George Sarre

AU - Peterse, Evert

AU - Schröder, Sybrin P

AU - Florea, Bogdan I.

AU - Jiang, Jianbing

AU - Reijngoud, Jos

AU - Ram, Arthur F.J.

AU - Van Der Marel, Gijsbert A.

AU - Van Wezel, Gilles P.

AU - Codee, Jeroen D. C.

AU - Overkleeft, Herman S.

AU - Davies, Gideon John

N1 - © The Royal Society of Chemistry 2020

PY - 2020/7/28

Y1 - 2020/7/28

N2 - Cellulases and related β-1,4-glucanases are essential components of lignocellulose-degrading enzyme mixtures. The detection of β-1,4-glucanase activity typically relies on monitoring the breakdown of purified lignocellulose-derived substrates or synthetic chromogenic substrates, limiting the activities which can be detected and complicating the tracing of activity back to specific components within complex enzyme mixtures. As a tool for the rapid detection and identification of β-1,4-glucanases, a series of glycosylated cyclophellitol inhibitors mimicking β-1,4-glucan oligosaccharides have been synthesised. These compounds are highly efficient inhibitors of HiCel7B, a well-known GH7 endo -β-1,4-glucanase. An elaborated activity-based probe facilitated the direct detection and identification of β-1,4-glucanases within a complex fungal secretome without any detectable cross-reactivity with β- d -glucosidases. These probes and inhibitors add valuable new capacity to the growing toolbox of cyclophellitol-derived probes for the activity-based profiling of biomass-degrading enzymes.

AB - Cellulases and related β-1,4-glucanases are essential components of lignocellulose-degrading enzyme mixtures. The detection of β-1,4-glucanase activity typically relies on monitoring the breakdown of purified lignocellulose-derived substrates or synthetic chromogenic substrates, limiting the activities which can be detected and complicating the tracing of activity back to specific components within complex enzyme mixtures. As a tool for the rapid detection and identification of β-1,4-glucanases, a series of glycosylated cyclophellitol inhibitors mimicking β-1,4-glucan oligosaccharides have been synthesised. These compounds are highly efficient inhibitors of HiCel7B, a well-known GH7 endo -β-1,4-glucanase. An elaborated activity-based probe facilitated the direct detection and identification of β-1,4-glucanases within a complex fungal secretome without any detectable cross-reactivity with β- d -glucosidases. These probes and inhibitors add valuable new capacity to the growing toolbox of cyclophellitol-derived probes for the activity-based profiling of biomass-degrading enzymes.

U2 - 10.1039/D0CB00045K

DO - 10.1039/D0CB00045K

M3 - Article

VL - 1

SP - 148

EP - 155

JO - RSC Chemical Biology

JF - RSC Chemical Biology

SN - 2633-0679

IS - 3

ER -