Guided mode resonance sensor for the parallel detection of multiple protein biomarkers in human urine with high sensitivity

TY - JOUR

T1 - Guided mode resonance sensor for the parallel detection of multiple protein biomarkers in human urine with high sensitivity

AU - Kenaan, Ahmad

AU - Li, Kezheng

AU - Barth, Isabel

AU - Johnson, Steven

AU - Song, Jie

AU - Krauss, Thomas F.

N1 - © 2020 Elsevier B.V. All rights reserved. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy.

PY - 2020/4/1

Y1 - 2020/4/1

N2 - The rising cost of global healthcare provision and new approaches to managing disease are driving the development of low-cost biosensing modalities, such as label-free photonic methods based on dielectric resonances. Here, we use the combined sensing and imaging capability of a guided mode resonance (GMR) sensor to detect multiple biomarkers (troponin, procalcitonin and C-Reactive Protein) in parallel in undiluted urine samples. A key requirement of such a biosensor is the simple and direct functionalization with suitable antibodies to ensure the disease-specific detection of protein biomarkers. Here, antibodies were immobilized using a succinimidyl-[(N-maleimidopropionamido)-hexaethyleneglycol] ester (SM(PEG)6) spacer. The polyethylene glycol (PEG) chemistry enables low detection limits of 10 pg mL-1 or better for all protein biomarkers, while minimizing non-specific binding compared to more commonly used strategies such as (3-Aminopropyl)triethoxysilane (APTES) or dextran. Our approach supports the vision of a simple yet highly sensitive diagnostic platform that could be used for pre-screening patients for a wide range of diseases at point-of-care, thereby relieving the pressure on overstretched healthcare services.

AB - The rising cost of global healthcare provision and new approaches to managing disease are driving the development of low-cost biosensing modalities, such as label-free photonic methods based on dielectric resonances. Here, we use the combined sensing and imaging capability of a guided mode resonance (GMR) sensor to detect multiple biomarkers (troponin, procalcitonin and C-Reactive Protein) in parallel in undiluted urine samples. A key requirement of such a biosensor is the simple and direct functionalization with suitable antibodies to ensure the disease-specific detection of protein biomarkers. Here, antibodies were immobilized using a succinimidyl-[(N-maleimidopropionamido)-hexaethyleneglycol] ester (SM(PEG)6) spacer. The polyethylene glycol (PEG) chemistry enables low detection limits of 10 pg mL-1 or better for all protein biomarkers, while minimizing non-specific binding compared to more commonly used strategies such as (3-Aminopropyl)triethoxysilane (APTES) or dextran. Our approach supports the vision of a simple yet highly sensitive diagnostic platform that could be used for pre-screening patients for a wide range of diseases at point-of-care, thereby relieving the pressure on overstretched healthcare services.

KW - Guided mode resonance

KW - Immunosensor

KW - Optical biosensor

KW - Polyethylene glycol

KW - Protein biomarkers

KW - Urine matrix

UR - http://www.scopus.com/inward/record.url?scp=85078462936&partnerID=8YFLogxK

U2 - 10.1016/j.bios.2020.112047

DO - 10.1016/j.bios.2020.112047

M3 - Article

C2 - 31999559

AN - SCOPUS:85078462936

SN - 0956-5663

VL - 153

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

M1 - 112047

ER -