Gut Bacterial Families Are Associated with Body Composition and Metabolic Risk Markers in School-Aged Children in Rural Mexico

Tania Aguilar; Gerardo M Nava; Andrea M Olvera-Ramírez; Dolores Ronquillo; Mariela Camacho; Gerardo A Zavala; María C Caamaño; Karina Acevedo-Whitehouse; Jorge L Rosado; Olga P García

doi:10.1089/chi.2019.0312

Gut Bacterial Families Are Associated with Body Composition and Metabolic Risk Markers in School-Aged Children in Rural Mexico

Tania Aguilar, Gerardo M Nava, Andrea M Olvera-Ramírez, Dolores Ronquillo, Mariela Camacho, Gerardo A Zavala, María C Caamaño, Karina Acevedo-Whitehouse, Jorge L Rosado, Olga P García

Health Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Background: Differences in gut microbiota composition have been associated with obesity and metabolic alterations in children. The aim of this study was to analyze the abundance of the main bacterial families of the gut among children according to their body composition and metabolic markers. Methods: A cross-sectional study was conducted with 93 school-aged children (8.4 ± 1.6 years old). Anthropometric and body composition variables were measured and a blood sample was collected to determine glucose, insulin, lipid profile, C-reactive protein, leptin, and cytokines [interleukin 6, interleukin 10 (IL-10), tumor necrosis factor α (TNFα)]. DNA was extracted from stool samples and the abundance of bacterial families (Bacteroidaceae-Porphyromonadaceae-Prevotellaceae, Lactobacillaceae, Enterococcaceae, and Lachnospiraceae-Ruminococcaceae) was determined by qPCR assays. Results: Children with obesity and high waist/height ratio had lower Bacteroidaceae-Porphyromonadaceae-Prevotellaceae and higher abundance of Lactobacillaceae when compared with normal-weight children. TNFα was negatively associated and IL-10 was positively associated with Bacteroidaceae-Porphyromonadaceae-Prevotellaceae. Triglycerides showed a positive relationship with Lachnospiraceae-Ruminococcaceae whereas high-density lipoprotein-cholesterol was negatively associated with Lactobacillaceae. Conclusion: In rural Mexican school-aged children, a low abundance of Bacteroidaceae-Porphyromonadaceae-Prevotellaceae and a high abundance of Lactobacillaceae are associated with obesity and metabolic disturbances.

Original language	English
Pages (from-to)	358-366
Number of pages	9
Journal	Childhood obesity (Print)
Volume	16
Issue number	5
DOIs	https://doi.org/10.1089/chi.2019.0312
Publication status	E-pub ahead of print - 19 May 2020

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Copyright 2020, Mary Ann Liebert, Inc., publishers. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details.

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10.1089/chi.2019.0312

Gut_bacterial_families_and_Obesity
Copyright 2020, Mary Ann Liebert, Inc., publishers. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details.
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Cite this

Aguilar, T., Nava, G. M., Olvera-Ramírez, A. M., Ronquillo, D., Camacho, M., Zavala, G. A., Caamaño, M. C., Acevedo-Whitehouse, K., Rosado, J. L., & García, O. P. (2020). Gut Bacterial Families Are Associated with Body Composition and Metabolic Risk Markers in School-Aged Children in Rural Mexico. Childhood obesity (Print), 16(5), 358-366. Advance online publication. https://doi.org/10.1089/chi.2019.0312

@article{ad135e06db054dac9f37c4ce5d9a288f,

title = "Gut Bacterial Families Are Associated with Body Composition and Metabolic Risk Markers in School-Aged Children in Rural Mexico",

abstract = " Background: Differences in gut microbiota composition have been associated with obesity and metabolic alterations in children. The aim of this study was to analyze the abundance of the main bacterial families of the gut among children according to their body composition and metabolic markers. Methods: A cross-sectional study was conducted with 93 school-aged children (8.4 ± 1.6 years old). Anthropometric and body composition variables were measured and a blood sample was collected to determine glucose, insulin, lipid profile, C-reactive protein, leptin, and cytokines [interleukin 6, interleukin 10 (IL-10), tumor necrosis factor α (TNFα)]. DNA was extracted from stool samples and the abundance of bacterial families (Bacteroidaceae-Porphyromonadaceae-Prevotellaceae, Lactobacillaceae, Enterococcaceae, and Lachnospiraceae-Ruminococcaceae) was determined by qPCR assays. Results: Children with obesity and high waist/height ratio had lower Bacteroidaceae-Porphyromonadaceae-Prevotellaceae and higher abundance of Lactobacillaceae when compared with normal-weight children. TNFα was negatively associated and IL-10 was positively associated with Bacteroidaceae-Porphyromonadaceae-Prevotellaceae. Triglycerides showed a positive relationship with Lachnospiraceae-Ruminococcaceae whereas high-density lipoprotein-cholesterol was negatively associated with Lactobacillaceae. Conclusion: In rural Mexican school-aged children, a low abundance of Bacteroidaceae-Porphyromonadaceae-Prevotellaceae and a high abundance of Lactobacillaceae are associated with obesity and metabolic disturbances.",

author = "Tania Aguilar and Nava, {Gerardo M} and Olvera-Ram{\'i}rez, {Andrea M} and Dolores Ronquillo and Mariela Camacho and Zavala, {Gerardo A} and Caama{\~n}o, {Mar{\'i}a C} and Karina Acevedo-Whitehouse and Rosado, {Jorge L} and Garc{\'i}a, {Olga P}",

note = "Copyright 2020, Mary Ann Liebert, Inc., publishers. This is an author-produced version of the published paper. Uploaded in accordance with the publisher{\textquoteright}s self-archiving policy. Further copying may not be permitted; contact the publisher for details.",

year = "2020",

month = may,

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doi = "10.1089/chi.2019.0312",

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Aguilar, T, Nava, GM, Olvera-Ramírez, AM, Ronquillo, D, Camacho, M, Zavala, GA, Caamaño, MC, Acevedo-Whitehouse, K, Rosado, JL & García, OP 2020, 'Gut Bacterial Families Are Associated with Body Composition and Metabolic Risk Markers in School-Aged Children in Rural Mexico', Childhood obesity (Print), vol. 16, no. 5, pp. 358-366. https://doi.org/10.1089/chi.2019.0312

TY - JOUR

T1 - Gut Bacterial Families Are Associated with Body Composition and Metabolic Risk Markers in School-Aged Children in Rural Mexico

AU - Aguilar, Tania

AU - Nava, Gerardo M

AU - Olvera-Ramírez, Andrea M

AU - Ronquillo, Dolores

AU - Camacho, Mariela

AU - Zavala, Gerardo A

AU - Caamaño, María C

AU - Acevedo-Whitehouse, Karina

AU - Rosado, Jorge L

AU - García, Olga P

N1 - Copyright 2020, Mary Ann Liebert, Inc., publishers. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details.

PY - 2020/5/19

Y1 - 2020/5/19

N2 - Background: Differences in gut microbiota composition have been associated with obesity and metabolic alterations in children. The aim of this study was to analyze the abundance of the main bacterial families of the gut among children according to their body composition and metabolic markers. Methods: A cross-sectional study was conducted with 93 school-aged children (8.4 ± 1.6 years old). Anthropometric and body composition variables were measured and a blood sample was collected to determine glucose, insulin, lipid profile, C-reactive protein, leptin, and cytokines [interleukin 6, interleukin 10 (IL-10), tumor necrosis factor α (TNFα)]. DNA was extracted from stool samples and the abundance of bacterial families (Bacteroidaceae-Porphyromonadaceae-Prevotellaceae, Lactobacillaceae, Enterococcaceae, and Lachnospiraceae-Ruminococcaceae) was determined by qPCR assays. Results: Children with obesity and high waist/height ratio had lower Bacteroidaceae-Porphyromonadaceae-Prevotellaceae and higher abundance of Lactobacillaceae when compared with normal-weight children. TNFα was negatively associated and IL-10 was positively associated with Bacteroidaceae-Porphyromonadaceae-Prevotellaceae. Triglycerides showed a positive relationship with Lachnospiraceae-Ruminococcaceae whereas high-density lipoprotein-cholesterol was negatively associated with Lactobacillaceae. Conclusion: In rural Mexican school-aged children, a low abundance of Bacteroidaceae-Porphyromonadaceae-Prevotellaceae and a high abundance of Lactobacillaceae are associated with obesity and metabolic disturbances.

AB - Background: Differences in gut microbiota composition have been associated with obesity and metabolic alterations in children. The aim of this study was to analyze the abundance of the main bacterial families of the gut among children according to their body composition and metabolic markers. Methods: A cross-sectional study was conducted with 93 school-aged children (8.4 ± 1.6 years old). Anthropometric and body composition variables were measured and a blood sample was collected to determine glucose, insulin, lipid profile, C-reactive protein, leptin, and cytokines [interleukin 6, interleukin 10 (IL-10), tumor necrosis factor α (TNFα)]. DNA was extracted from stool samples and the abundance of bacterial families (Bacteroidaceae-Porphyromonadaceae-Prevotellaceae, Lactobacillaceae, Enterococcaceae, and Lachnospiraceae-Ruminococcaceae) was determined by qPCR assays. Results: Children with obesity and high waist/height ratio had lower Bacteroidaceae-Porphyromonadaceae-Prevotellaceae and higher abundance of Lactobacillaceae when compared with normal-weight children. TNFα was negatively associated and IL-10 was positively associated with Bacteroidaceae-Porphyromonadaceae-Prevotellaceae. Triglycerides showed a positive relationship with Lachnospiraceae-Ruminococcaceae whereas high-density lipoprotein-cholesterol was negatively associated with Lactobacillaceae. Conclusion: In rural Mexican school-aged children, a low abundance of Bacteroidaceae-Porphyromonadaceae-Prevotellaceae and a high abundance of Lactobacillaceae are associated with obesity and metabolic disturbances.

U2 - 10.1089/chi.2019.0312

DO - 10.1089/chi.2019.0312

M3 - Article

C2 - 32429742

SN - 2153-2168

VL - 16

SP - 358

EP - 366

JO - Childhood obesity (Print)

JF - Childhood obesity (Print)

IS - 5

ER -