Harnessing glycofluoroforms for impedimetric biosensing

TY - JOUR

T1 - Harnessing glycofluoroforms for impedimetric biosensing

AU - Hewson, Alice

AU - Lloyd-Laney, Henry

AU - Keenan, Tessa

AU - Richards, Sarah-Jane

AU - Gibson, Matthew I.

AU - Linclau, Bruno

AU - Signoret, Nathalie Yvonne Micheline

AU - Fascione, Martin Anthony

AU - Parkin, Alison

N1 - © 2024 The Royal Society of Chemistry.

PY - 2024/9/13

Y1 - 2024/9/13

N2 - Glycans play a major role in biological cell-cell recognition and signal transduction but have found limited application in biosensors due to glycan/lectin promiscuity; multiple proteins are capable of binding to the same native glycan. Here, site-specific fluorination is used to introduce protein-glycan selectivity, and this is coupled with an electrochemical detection method to generate a novel biosensor platform. 3F-lacto-N-biose glycofluoroform is installed onto polymer tethers, which are subsequently immobilised onto gold screen printed electrodes, providing a non-fouling surface. The impedance biosensing platform is shown to selectively bind cancer-associated galectin-3 compared to control glycans and proteins. To improve the analytical capability, Bayesian statistical analysis was deployed in the equivalent circuit fitting of electrochemical impedance spectroscopy data. It is shown that Markov Chain Monte Carlo (MCMC) analysis is a helpful method for visualising experimental irreproducibility, and we apply this as a quality control step.

AB - Glycans play a major role in biological cell-cell recognition and signal transduction but have found limited application in biosensors due to glycan/lectin promiscuity; multiple proteins are capable of binding to the same native glycan. Here, site-specific fluorination is used to introduce protein-glycan selectivity, and this is coupled with an electrochemical detection method to generate a novel biosensor platform. 3F-lacto-N-biose glycofluoroform is installed onto polymer tethers, which are subsequently immobilised onto gold screen printed electrodes, providing a non-fouling surface. The impedance biosensing platform is shown to selectively bind cancer-associated galectin-3 compared to control glycans and proteins. To improve the analytical capability, Bayesian statistical analysis was deployed in the equivalent circuit fitting of electrochemical impedance spectroscopy data. It is shown that Markov Chain Monte Carlo (MCMC) analysis is a helpful method for visualising experimental irreproducibility, and we apply this as a quality control step.

UR - http://www.scopus.com/inward/record.url?scp=85204372970&partnerID=8YFLogxK

U2 - 10.1039/d4sc04409f

DO - 10.1039/d4sc04409f

M3 - Article

AN - SCOPUS:85204372970

SN - 2041-6520

VL - 15

SP - 16086

EP - 16095

JO - Chemical Science

JF - Chemical Science

IS - 39

ER -