Harvesting Human Prostate Tissue Material and Culturing Primary Prostate Epithelial Cells

Fiona M Frame; Davide Pellacani; Anne T Collins; Norman J Maitland

doi:10.1007/978-1-4939-3724-0_12

Harvesting Human Prostate Tissue Material and Culturing Primary Prostate Epithelial Cells

Fiona M Frame, Davide Pellacani, Anne T Collins, Norman J Maitland

Research output: Contribution to journal › Article › peer-review

Abstract

In order to fully explore the biology of a complex solid tumor such as prostate cancer, it is desirable to work with patient tissue. Only by working with cells from a tissue can we take into account patient variability and tumor heterogeneity. Cell lines have long been regarded as the workhorse of cancer research and it could be argued that they are of most use when considered within a panel of cell lines, thus taking into account specified mutations and variations in phenotype between different cell lines. However, often very different results are obtained when comparing cell lines to primary cells cultured from tissue. It stands to reason that cells cultured from patient tissue represents a close-to-patient model that should and does produce clinically relevant data. This chapter aims to illustrate the methods of processing, storing and culturing cells from prostate tissue, with a description of potential uses.

Original language	English
Pages (from-to)	181-201
Number of pages	21
Journal	Methods in Molecular Biology
Volume	1443
DOIs	https://doi.org/10.1007/978-1-4939-3724-0_12
Publication status	Published - 1 Jun 2016

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10.1007/978-1-4939-3724-0_12

Cite this

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title = "Harvesting Human Prostate Tissue Material and Culturing Primary Prostate Epithelial Cells",

abstract = "In order to fully explore the biology of a complex solid tumor such as prostate cancer, it is desirable to work with patient tissue. Only by working with cells from a tissue can we take into account patient variability and tumor heterogeneity. Cell lines have long been regarded as the workhorse of cancer research and it could be argued that they are of most use when considered within a panel of cell lines, thus taking into account specified mutations and variations in phenotype between different cell lines. However, often very different results are obtained when comparing cell lines to primary cells cultured from tissue. It stands to reason that cells cultured from patient tissue represents a close-to-patient model that should and does produce clinically relevant data. This chapter aims to illustrate the methods of processing, storing and culturing cells from prostate tissue, with a description of potential uses.",

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