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Harvesting Human Prostate Tissue Material and Culturing Primary Prostate Epithelial Cells

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JournalMethods in Molecular Biology
DateAccepted/In press - 9 Mar 2016
DatePublished (current) - 1 Jun 2016
Volume1443
Number of pages21
Pages (from-to)181-201
Original languageEnglish

Abstract

In order to fully explore the biology of a complex solid tumor such as prostate cancer, it is desirable to work with patient tissue. Only by working with cells from a tissue can we take into account patient variability and tumor heterogeneity. Cell lines have long been regarded as the workhorse of cancer research and it could be argued that they are of most use when considered within a panel of cell lines, thus taking into account specified mutations and variations in phenotype between different cell lines. However, often very different results are obtained when comparing cell lines to primary cells cultured from tissue. It stands to reason that cells cultured from patient tissue represents a close-to-patient model that should and does produce clinically relevant data. This chapter aims to illustrate the methods of processing, storing and culturing cells from prostate tissue, with a description of potential uses.

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