TY - JOUR
T1 - High-resolution live cell imaging to define ultrastructural and dynamic features of the halotolerant yeast Debaryomyces hansenii
AU - Xelhuantzi, Martha S.C.
AU - Ghete, Daniel
AU - Milburn, Amy
AU - Ioannou, Savvas
AU - Mudd, Phoebe
AU - Calder, Grant
AU - Ramos, José
AU - O’Toole, Peter J.
AU - Genever, Paul G.
AU - MacDonald, Chris
N1 - © 2024. Published by The Company of Biologists Ltd
PY - 2024/7/30
Y1 - 2024/7/30
N2 - Although some budding yeasts have proved tractable and intensely studied models, others are more recalcitrant. Debaryomyces hansenii, an important yeast species in food and biotechnological industries with curious physiological characteristics, has proved difficult to manipulate genetically and remains poorly defined. To remedy this, we have combined live cell fluorescent dyes with high-resolution imaging techniques to define the sub-cellular features of D. hansenii, such as the mitochondria, nuclei, vacuoles and the cell wall. Using these tools, we define biological processes like the cell cycle, organelle inheritance and various membrane trafficking pathways of D. hansenii for the first time. Beyond this, reagents designed to study Saccharomyces cerevisiae proteins were used to access proteomic information about D. hansenii. Finally, we optimised the use of label-free holotomography to image yeast, defining the physical parameters and visualising sub-cellular features like membranes and vacuoles. Not only does this work shed light on D. hansenii but this combinatorial approach serves as a template for how other cell biological systems, which are not amenable to standard genetic procedures, can be studied.
AB - Although some budding yeasts have proved tractable and intensely studied models, others are more recalcitrant. Debaryomyces hansenii, an important yeast species in food and biotechnological industries with curious physiological characteristics, has proved difficult to manipulate genetically and remains poorly defined. To remedy this, we have combined live cell fluorescent dyes with high-resolution imaging techniques to define the sub-cellular features of D. hansenii, such as the mitochondria, nuclei, vacuoles and the cell wall. Using these tools, we define biological processes like the cell cycle, organelle inheritance and various membrane trafficking pathways of D. hansenii for the first time. Beyond this, reagents designed to study Saccharomyces cerevisiae proteins were used to access proteomic information about D. hansenii. Finally, we optimised the use of label-free holotomography to image yeast, defining the physical parameters and visualising sub-cellular features like membranes and vacuoles. Not only does this work shed light on D. hansenii but this combinatorial approach serves as a template for how other cell biological systems, which are not amenable to standard genetic procedures, can be studied.
KW - Debaryomyces hansenii
KW - Holotomography
KW - Membrane trafficking
KW - Organelle organisation
KW - Super resolution microscopy
UR - http://www.scopus.com/inward/record.url?scp=85201214056&partnerID=8YFLogxK
U2 - 10.1242/bio.060519
DO - 10.1242/bio.060519
M3 - Article
AN - SCOPUS:85201214056
SN - 2046-6390
VL - 13
JO - Biology Open
JF - Biology Open
IS - 7
M1 - bio060519
ER -