High-resolution mapping of transcriptional dynamics across tissue development reveals a stable mRNA-tRNA interface

TY - JOUR

T1 - High-resolution mapping of transcriptional dynamics across tissue development reveals a stable mRNA-tRNA interface

AU - Schmitt, Bianca M.

AU - Rudolph, Konrad L. M.

AU - Karagianni, Panagiota

AU - Fonseca, Nuno A.

AU - White, Robert J.

AU - Talianidis, Iannis

AU - Odom, Duncan T.

AU - Marioni, John C.

AU - Kutter, Claudia

N1 - © 2014 Schmitt et al.; Published by Cold Spring Harbor Laboratory Press.

PY - 2014/11

Y1 - 2014/11

N2 - The genetic code is an abstraction of how mRNA codons and tRNA anticodons molecularly interact during protein synthesis; the stability and regulation of this interaction remains largely unexplored. Here, we characterized the expression of mRNA and tRNA genes quantitatively at multiple time points in two developing mouse tissues. We discovered that mRNA codon pools are highly stable over development and simply reflect the genomic background; in contrast, precise regulation of tRNA gene families is required to create the corresponding tRNA transcriptomes. The dynamic regulation of tRNA genes during development is controlled in order to generate an anticodon pool that closely corresponds to messenger RNAs. Thus, across development, the pools of mRNA codons and tRNA anticodons are invariant and highly correlated, revealing a stable molecular interaction interlocking transcription and translation.

AB - The genetic code is an abstraction of how mRNA codons and tRNA anticodons molecularly interact during protein synthesis; the stability and regulation of this interaction remains largely unexplored. Here, we characterized the expression of mRNA and tRNA genes quantitatively at multiple time points in two developing mouse tissues. We discovered that mRNA codon pools are highly stable over development and simply reflect the genomic background; in contrast, precise regulation of tRNA gene families is required to create the corresponding tRNA transcriptomes. The dynamic regulation of tRNA genes during development is controlled in order to generate an anticodon pool that closely corresponds to messenger RNAs. Thus, across development, the pools of mRNA codons and tRNA anticodons are invariant and highly correlated, revealing a stable molecular interaction interlocking transcription and translation.

U2 - 10.1101/gr.176784.114

DO - 10.1101/gr.176784.114

M3 - Article

C2 - 25122613

SN - 1088-9051

VL - 24

SP - 1797

EP - 1807

JO - Genome Research

JF - Genome Research

IS - 11

ER -