High-throughput identification of transient extracellular protein interactions.: Biochemical Society Transactions

Gavin J Wright; Stephen Martin; K Mark Bushell; Christian Söllner

doi:10.1042/BST0380919

High-throughput identification of transient extracellular protein interactions. Biochemical Society Transactions

Gavin J Wright, Stephen Martin, K Mark Bushell, Christian Söllner

Research output: Contribution to journal › Article › peer-review

Abstract

Protein interactions are highly diverse in their biochemical nature, varying in affinity and are often dependent on the surrounding biochemical environment. Given this heterogeneity, it seems unlikely that any one method, and particularly those capable of screening for many protein interactions in parallel, will be able to detect all functionally relevant interactions that occur within a living cell. One major class of interactions that are not detected by current popular high-throughput methods are those that occur in the extracellular environment, especially those made by membrane-embedded receptor proteins. In the present article, we discuss some of our recent research in the development of a scalable assay to identify this class of protein interaction and some of the findings from its application in the construction of extracellular protein interaction networks.

Original language	English
Pages (from-to)	919-922
Number of pages	4
Journal	Biochem Soc Trans
Volume	38
Issue number	4
DOIs	https://doi.org/10.1042/BST0380919
Publication status	Published - 2010

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10.1042/BST0380919

Cite this

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abstract = "Protein interactions are highly diverse in their biochemical nature, varying in affinity and are often dependent on the surrounding biochemical environment. Given this heterogeneity, it seems unlikely that any one method, and particularly those capable of screening for many protein interactions in parallel, will be able to detect all functionally relevant interactions that occur within a living cell. One major class of interactions that are not detected by current popular high-throughput methods are those that occur in the extracellular environment, especially those made by membrane-embedded receptor proteins. In the present article, we discuss some of our recent research in the development of a scalable assay to identify this class of protein interaction and some of the findings from its application in the construction of extracellular protein interaction networks.",

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