High-Throughput Peptide Derivatization toward Supramolecular Diversification in Microtiter Plates

Yiyang Lin; Matthew Penna; Christopher D Spicer; Stuart G Higgins; Amy Gelmi; Nayoung Kim; Shih-Ting Wang; Jonathan P Wojciechowski; E Thomas Pashuck; Irene Yarovsky; Molly M Stevens

doi:10.1021/acsnano.0c05423

High-Throughput Peptide Derivatization toward Supramolecular Diversification in Microtiter Plates

Yiyang Lin, Matthew Penna, Christopher D Spicer, Stuart G Higgins, Amy Gelmi, Nayoung Kim, Shih-Ting Wang, Jonathan P Wojciechowski, E Thomas Pashuck, Irene Yarovsky, Molly M Stevens

Research output: Contribution to journal › Article › peer-review

Abstract

The evolution of life on earth eventually leads to the emergence of species with increased complexity and diversity. Similarly, evolutionary chemical space exploration in the laboratory is a key step to pursue the structural and functional diversity of supramolecular systems. Here, we present a powerful tool that enables rapid peptide diversification and employ it to expand the chemical space for supramolecular functions. Central to this strategy is the exploitation of palladium-catalyzed Suzuki-Miyaura cross-coupling reactions to direct combinatorial synthesis of peptide arrays in microtiter plates under an open atmosphere. Taking advantage of this in situ library design, our results unambiguously deliver a fertile platform for creating a set of intriguing peptide functions including green fluorescent protein-like peptide emitters with chemically encoded emission colors, hierarchical self-assembly into nano-objects, and macroscopic hydrogels. This work also offers opportunities for quickly surveying the diversified peptide arrays and thereby identifying the structural factors that modulate peptide properties.

Original language	English
Pages (from-to)	4034-4044
Number of pages	11
Journal	ACS Nano
Volume	15
Issue number	3
Early online date	15 Feb 2021
DOIs	https://doi.org/10.1021/acsnano.0c05423
Publication status	Published - 23 Mar 2021

Bibliographical note

Keywords

Hydrogels
Palladium
Peptides

Access to Document

10.1021/acsnano.0c05423Licence: CC BY

acsnano.0c05423
© 2021 American Chemical Society
Final published version, 9.33 MBLicence: CC BY

Cite this

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title = "High-Throughput Peptide Derivatization toward Supramolecular Diversification in Microtiter Plates",

abstract = "The evolution of life on earth eventually leads to the emergence of species with increased complexity and diversity. Similarly, evolutionary chemical space exploration in the laboratory is a key step to pursue the structural and functional diversity of supramolecular systems. Here, we present a powerful tool that enables rapid peptide diversification and employ it to expand the chemical space for supramolecular functions. Central to this strategy is the exploitation of palladium-catalyzed Suzuki-Miyaura cross-coupling reactions to direct combinatorial synthesis of peptide arrays in microtiter plates under an open atmosphere. Taking advantage of this in situ library design, our results unambiguously deliver a fertile platform for creating a set of intriguing peptide functions including green fluorescent protein-like peptide emitters with chemically encoded emission colors, hierarchical self-assembly into nano-objects, and macroscopic hydrogels. This work also offers opportunities for quickly surveying the diversified peptide arrays and thereby identifying the structural factors that modulate peptide properties.",

keywords = "Hydrogels, Palladium, Peptides",

author = "Yiyang Lin and Matthew Penna and Spicer, {Christopher D} and Higgins, {Stuart G} and Amy Gelmi and Nayoung Kim and Shih-Ting Wang and Wojciechowski, {Jonathan P} and Pashuck, {E Thomas} and Irene Yarovsky and Stevens, {Molly M}",

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T1 - High-Throughput Peptide Derivatization toward Supramolecular Diversification in Microtiter Plates

AU - Lin, Yiyang

AU - Penna, Matthew

AU - Spicer, Christopher D

AU - Higgins, Stuart G

AU - Gelmi, Amy

AU - Kim, Nayoung

AU - Wang, Shih-Ting

AU - Wojciechowski, Jonathan P

AU - Pashuck, E Thomas

AU - Yarovsky, Irene

AU - Stevens, Molly M

PY - 2021/3/23

Y1 - 2021/3/23

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AB - The evolution of life on earth eventually leads to the emergence of species with increased complexity and diversity. Similarly, evolutionary chemical space exploration in the laboratory is a key step to pursue the structural and functional diversity of supramolecular systems. Here, we present a powerful tool that enables rapid peptide diversification and employ it to expand the chemical space for supramolecular functions. Central to this strategy is the exploitation of palladium-catalyzed Suzuki-Miyaura cross-coupling reactions to direct combinatorial synthesis of peptide arrays in microtiter plates under an open atmosphere. Taking advantage of this in situ library design, our results unambiguously deliver a fertile platform for creating a set of intriguing peptide functions including green fluorescent protein-like peptide emitters with chemically encoded emission colors, hierarchical self-assembly into nano-objects, and macroscopic hydrogels. This work also offers opportunities for quickly surveying the diversified peptide arrays and thereby identifying the structural factors that modulate peptide properties.

KW - Hydrogels

KW - Palladium

KW - Peptides

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