Higher diversity of Rhizobium leguminosarum biovar viciae populations in arable soils than in grass soils

K M Palmer; J P W Young

Higher diversity of Rhizobium leguminosarum biovar viciae populations in arable soils than in grass soils

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

The bacterial genetic diversity after long-term arable cultivation was compared with that under permanent grassland using replicated paired contrasts, Pea-nodulating Rhizobium leguminosarum populations were sampled from pairs of arable and grass sites at four locations in Yorkshire, United Kingdom, isolates were characterized using both chromosomal (16S-23S ribosomal DNA internal transcribed spacer PCR-restriction fragment length polymorphism) and plasmid (group-specific repC PCR amplification) markers. The diversities of chromosomal types, repC profiles, and combined genotypes were calculated using richness in types (adjusted to equal sample sizes by rarefaction), Shannon-Wiener index, and Simpson's index. The relative differences in diversity within each pair of sites were similar for all three diversity measures, Chromosomal types, repC profiles, and combined genotypes were each more diverse in arable soils than in grass soils at two of the four locations. The other comparisons showed no significant differences. We conclude that rhizobial diversity can be affected by differences between these two management regimens. Multiple regression analyses indicated that lower diversity was associated with high potential nitrogen and phosphate levels or with acidity.

Original language	English
Pages (from-to)	2445-2450
Number of pages	6
Journal	Applied and Environmental Microbiology
Volume	66
Issue number	6
Publication status	Published - Jun 2000

Bibliographical note

Keywords

PLASMID-REPLICATION SEQUENCES
GENETIC DIVERSITY
BV-TRIFOLII
ENZYME POLYMORPHISM
FIELD POPULATIONS

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title = "Higher diversity of Rhizobium leguminosarum biovar viciae populations in arable soils than in grass soils",

abstract = "The bacterial genetic diversity after long-term arable cultivation was compared with that under permanent grassland using replicated paired contrasts, Pea-nodulating Rhizobium leguminosarum populations were sampled from pairs of arable and grass sites at four locations in Yorkshire, United Kingdom, isolates were characterized using both chromosomal (16S-23S ribosomal DNA internal transcribed spacer PCR-restriction fragment length polymorphism) and plasmid (group-specific repC PCR amplification) markers. The diversities of chromosomal types, repC profiles, and combined genotypes were calculated using richness in types (adjusted to equal sample sizes by rarefaction), Shannon-Wiener index, and Simpson's index. The relative differences in diversity within each pair of sites were similar for all three diversity measures, Chromosomal types, repC profiles, and combined genotypes were each more diverse in arable soils than in grass soils at two of the four locations. The other comparisons showed no significant differences. We conclude that rhizobial diversity can be affected by differences between these two management regimens. Multiple regression analyses indicated that lower diversity was associated with high potential nitrogen and phosphate levels or with acidity.",

keywords = "PLASMID-REPLICATION SEQUENCES, GENETIC DIVERSITY, BV-TRIFOLII, ENZYME POLYMORPHISM, FIELD POPULATIONS",

author = "Palmer, {K M} and Young, {J P W}",

note = "Copyright {\textcopyright} 2000 American Society for Microbiology",

year = "2000",

month = jun,

language = "English",

volume = "66",

pages = "2445--2450",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

number = "6",

}

TY - JOUR

T1 - Higher diversity of Rhizobium leguminosarum biovar viciae populations in arable soils than in grass soils

AU - Palmer, K M

AU - Young, J P W

PY - 2000/6

Y1 - 2000/6

N2 - The bacterial genetic diversity after long-term arable cultivation was compared with that under permanent grassland using replicated paired contrasts, Pea-nodulating Rhizobium leguminosarum populations were sampled from pairs of arable and grass sites at four locations in Yorkshire, United Kingdom, isolates were characterized using both chromosomal (16S-23S ribosomal DNA internal transcribed spacer PCR-restriction fragment length polymorphism) and plasmid (group-specific repC PCR amplification) markers. The diversities of chromosomal types, repC profiles, and combined genotypes were calculated using richness in types (adjusted to equal sample sizes by rarefaction), Shannon-Wiener index, and Simpson's index. The relative differences in diversity within each pair of sites were similar for all three diversity measures, Chromosomal types, repC profiles, and combined genotypes were each more diverse in arable soils than in grass soils at two of the four locations. The other comparisons showed no significant differences. We conclude that rhizobial diversity can be affected by differences between these two management regimens. Multiple regression analyses indicated that lower diversity was associated with high potential nitrogen and phosphate levels or with acidity.

AB - The bacterial genetic diversity after long-term arable cultivation was compared with that under permanent grassland using replicated paired contrasts, Pea-nodulating Rhizobium leguminosarum populations were sampled from pairs of arable and grass sites at four locations in Yorkshire, United Kingdom, isolates were characterized using both chromosomal (16S-23S ribosomal DNA internal transcribed spacer PCR-restriction fragment length polymorphism) and plasmid (group-specific repC PCR amplification) markers. The diversities of chromosomal types, repC profiles, and combined genotypes were calculated using richness in types (adjusted to equal sample sizes by rarefaction), Shannon-Wiener index, and Simpson's index. The relative differences in diversity within each pair of sites were similar for all three diversity measures, Chromosomal types, repC profiles, and combined genotypes were each more diverse in arable soils than in grass soils at two of the four locations. The other comparisons showed no significant differences. We conclude that rhizobial diversity can be affected by differences between these two management regimens. Multiple regression analyses indicated that lower diversity was associated with high potential nitrogen and phosphate levels or with acidity.

KW - PLASMID-REPLICATION SEQUENCES

KW - GENETIC DIVERSITY

KW - BV-TRIFOLII

KW - ENZYME POLYMORPHISM

KW - FIELD POPULATIONS

M3 - Article

VL - 66

SP - 2445

EP - 2450

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 6

ER -