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From the same journal

HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF LOW-LEVELS OF PRIMARY AND SECONDARY-AMINES IN AQUEOUS-SOLUTIONS INCLUDING 2-AMINO-2-METHYLPROPANOL BY PRECOLUMN DERIVATIZATION TO SULFONAMIDES

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Publication details

JournalJournal of chromatography
DatePublished - 28 Jun 1991
Issue number1-2
Volume547
Number of pages5
Pages (from-to)447-451
Original languageEnglish

Abstract

Pre-column derivatisation of primary and secondary amines to sulphonamides, by reaction with 5-dimethylamino-1-naphthalenesulphonyl chloride (dansyl chloride) has advantages over the corresponding derivatisation with 1,2-naphthoquinone-4-sulphonate (NQS) because the reaction is less susceptible to steric effects, and the resultant sulphonamides are readily extracted from the derivatising solution. A procedure employing a relatively high reaction temperature and chloroform extraction followed by reversed-phase high-performance liquid chromatography using ultraviolet detection at 340 nm provides a robust method for determining primary and secondary amines. The method was used to analyse samples of industrial waters containing various mixtures (0-2 mM) of ammonia, hexamethyleneimine, hydrazine, methylamine and piperidine, as well as 2-amino-2-methylpropanol and 1,6-hexanediamine that could not be analysed via NQS derivatisation.

    Research areas

  • DERIVATIVES, POLYAMINES

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