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HOST-SPECIFICITY OF RHIZOBIUM-LEGUMINOSARUM IS DETERMINED BY THE HYDROPHOBICITY OF HIGHLY UNSATURATED PATTY ACYL MOIETIES OF THE NODULATION FACTORS

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HOST-SPECIFICITY OF RHIZOBIUM-LEGUMINOSARUM IS DETERMINED BY THE HYDROPHOBICITY OF HIGHLY UNSATURATED PATTY ACYL MOIETIES OF THE NODULATION FACTORS. / SPAINK, H P ; BLOEMBERG, G V ; VANBRUSSEL, A A N ; LUGTENBERG, B J J ; VANDERDRIFT, K M G M ; HAVERKAMP, J ; Thomas-Oates, Jane Elizabeth.

In: MOLECULAR PLANT-MICROBE INTERACTIONS, Vol. 8, No. 1, 1995, p. 155-164.

Research output: Contribution to journalArticle

Harvard

SPAINK, HP, BLOEMBERG, GV, VANBRUSSEL, AAN, LUGTENBERG, BJJ, VANDERDRIFT, KMGM, HAVERKAMP, J & Thomas-Oates, JE 1995, 'HOST-SPECIFICITY OF RHIZOBIUM-LEGUMINOSARUM IS DETERMINED BY THE HYDROPHOBICITY OF HIGHLY UNSATURATED PATTY ACYL MOIETIES OF THE NODULATION FACTORS', MOLECULAR PLANT-MICROBE INTERACTIONS, vol. 8, no. 1, pp. 155-164.

APA

SPAINK, H. P., BLOEMBERG, G. V., VANBRUSSEL, A. A. N., LUGTENBERG, B. J. J., VANDERDRIFT, K. M. G. M., HAVERKAMP, J., & Thomas-Oates, J. E. (1995). HOST-SPECIFICITY OF RHIZOBIUM-LEGUMINOSARUM IS DETERMINED BY THE HYDROPHOBICITY OF HIGHLY UNSATURATED PATTY ACYL MOIETIES OF THE NODULATION FACTORS. MOLECULAR PLANT-MICROBE INTERACTIONS, 8(1), 155-164.

Vancouver

SPAINK HP, BLOEMBERG GV, VANBRUSSEL AAN, LUGTENBERG BJJ, VANDERDRIFT KMGM, HAVERKAMP J et al. HOST-SPECIFICITY OF RHIZOBIUM-LEGUMINOSARUM IS DETERMINED BY THE HYDROPHOBICITY OF HIGHLY UNSATURATED PATTY ACYL MOIETIES OF THE NODULATION FACTORS. MOLECULAR PLANT-MICROBE INTERACTIONS. 1995;8(1):155-164.

Author

SPAINK, H P ; BLOEMBERG, G V ; VANBRUSSEL, A A N ; LUGTENBERG, B J J ; VANDERDRIFT, K M G M ; HAVERKAMP, J ; Thomas-Oates, Jane Elizabeth. / HOST-SPECIFICITY OF RHIZOBIUM-LEGUMINOSARUM IS DETERMINED BY THE HYDROPHOBICITY OF HIGHLY UNSATURATED PATTY ACYL MOIETIES OF THE NODULATION FACTORS. In: MOLECULAR PLANT-MICROBE INTERACTIONS. 1995 ; Vol. 8, No. 1. pp. 155-164.

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@article{064b0f671647499190d3299d1f7aa6b5,
title = "HOST-SPECIFICITY OF RHIZOBIUM-LEGUMINOSARUM IS DETERMINED BY THE HYDROPHOBICITY OF HIGHLY UNSATURATED PATTY ACYL MOIETIES OF THE NODULATION FACTORS",
abstract = "The nodE genes of Rhizobium leguminosarum bvs, trifolii and viciae strains are the major determinants of the host range of nodulation, Using mass spectrometry we have analyzed the structures of the major and minor lipooligosaccharides produced by two R, leguminosarum strains that differ only in the origin of the nod genes, The strain containing the nod genes of R. l. by. viciae produces lipo-chitin oligosaccharide (LCO) molecules that contain the common cis-vaccenyl (C18:1) or a highly unsaturated acyl (C18:4) group that is determined by nodE (Spaink et al, Nature 354:125-130, 1991), Here we show that, in addition, minor quantities of analogous molecules that contain different common fatty acyl groups such as stearyl (C18:0), palmityl (C16:0), and palmitoyl (C16:1) groups are produced, The set of LCOs produced by the strain containing the R. l. by. trifolii nod genes is similar, but major differences are found in the length and unsaturation of the fatty acyl groups of various LCOs. The R. l, by. trifolii strain does not produce LCO molecules that contain the C18:4 fatty acyl group. Instead, a complex mixture of other unsaturated fatty acyl groups containing either two, three, or four double bonds is produced, These novel fatty acyl groups are more hydrophobic than the C18:4 fatty acyl group found in by. viciae, as judged by highperformance liquid chromatography retention times, probably as a result of the longer carbon chain length, the lack of a cis double bond, or both, Another difference is that the relative amount of highly unsaturated fatty acid-containing LCOs produced by R. l. by. trifolii is much smaller An isogenic R. l. by. trifolii strain containing a Tn5 insertion in the nodE gene only produces LCOs containing the common fatty acyl groups and not the highly unsaturated types, The results indicate that the difference in the host range of the R. l. bvs, viciae and trifolii is determined by the overall hydrophobicity of the highly unsaturated fatty acyl moieties of LCOs rather than by a specific structural feature.",
keywords = "CHITIN, MASS SPECTROMETRY, SIGNAL MOLECULES, LIPO-OLIGOSACCHARIDE SIGNALS, SYM-PLASMID, ROOT NODULE, GENES, MELILOTI, TRIFOLII, CLOVER, IDENTIFICATION, RECOGNITION, EXPRESSION",
author = "SPAINK, {H P} and BLOEMBERG, {G V} and VANBRUSSEL, {A A N} and LUGTENBERG, {B J J} and VANDERDRIFT, {K M G M} and J HAVERKAMP and Thomas-Oates, {Jane Elizabeth}",
year = "1995",
language = "English",
volume = "8",
pages = "155--164",
journal = "MOLECULAR PLANT-MICROBE INTERACTIONS",
issn = "0894-0282",
publisher = "American Phytopathological Society",
number = "1",

}

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TY - JOUR

T1 - HOST-SPECIFICITY OF RHIZOBIUM-LEGUMINOSARUM IS DETERMINED BY THE HYDROPHOBICITY OF HIGHLY UNSATURATED PATTY ACYL MOIETIES OF THE NODULATION FACTORS

AU - SPAINK, H P

AU - BLOEMBERG, G V

AU - VANBRUSSEL, A A N

AU - LUGTENBERG, B J J

AU - VANDERDRIFT, K M G M

AU - HAVERKAMP, J

AU - Thomas-Oates, Jane Elizabeth

PY - 1995

Y1 - 1995

N2 - The nodE genes of Rhizobium leguminosarum bvs, trifolii and viciae strains are the major determinants of the host range of nodulation, Using mass spectrometry we have analyzed the structures of the major and minor lipooligosaccharides produced by two R, leguminosarum strains that differ only in the origin of the nod genes, The strain containing the nod genes of R. l. by. viciae produces lipo-chitin oligosaccharide (LCO) molecules that contain the common cis-vaccenyl (C18:1) or a highly unsaturated acyl (C18:4) group that is determined by nodE (Spaink et al, Nature 354:125-130, 1991), Here we show that, in addition, minor quantities of analogous molecules that contain different common fatty acyl groups such as stearyl (C18:0), palmityl (C16:0), and palmitoyl (C16:1) groups are produced, The set of LCOs produced by the strain containing the R. l. by. trifolii nod genes is similar, but major differences are found in the length and unsaturation of the fatty acyl groups of various LCOs. The R. l, by. trifolii strain does not produce LCO molecules that contain the C18:4 fatty acyl group. Instead, a complex mixture of other unsaturated fatty acyl groups containing either two, three, or four double bonds is produced, These novel fatty acyl groups are more hydrophobic than the C18:4 fatty acyl group found in by. viciae, as judged by highperformance liquid chromatography retention times, probably as a result of the longer carbon chain length, the lack of a cis double bond, or both, Another difference is that the relative amount of highly unsaturated fatty acid-containing LCOs produced by R. l. by. trifolii is much smaller An isogenic R. l. by. trifolii strain containing a Tn5 insertion in the nodE gene only produces LCOs containing the common fatty acyl groups and not the highly unsaturated types, The results indicate that the difference in the host range of the R. l. bvs, viciae and trifolii is determined by the overall hydrophobicity of the highly unsaturated fatty acyl moieties of LCOs rather than by a specific structural feature.

AB - The nodE genes of Rhizobium leguminosarum bvs, trifolii and viciae strains are the major determinants of the host range of nodulation, Using mass spectrometry we have analyzed the structures of the major and minor lipooligosaccharides produced by two R, leguminosarum strains that differ only in the origin of the nod genes, The strain containing the nod genes of R. l. by. viciae produces lipo-chitin oligosaccharide (LCO) molecules that contain the common cis-vaccenyl (C18:1) or a highly unsaturated acyl (C18:4) group that is determined by nodE (Spaink et al, Nature 354:125-130, 1991), Here we show that, in addition, minor quantities of analogous molecules that contain different common fatty acyl groups such as stearyl (C18:0), palmityl (C16:0), and palmitoyl (C16:1) groups are produced, The set of LCOs produced by the strain containing the R. l. by. trifolii nod genes is similar, but major differences are found in the length and unsaturation of the fatty acyl groups of various LCOs. The R. l, by. trifolii strain does not produce LCO molecules that contain the C18:4 fatty acyl group. Instead, a complex mixture of other unsaturated fatty acyl groups containing either two, three, or four double bonds is produced, These novel fatty acyl groups are more hydrophobic than the C18:4 fatty acyl group found in by. viciae, as judged by highperformance liquid chromatography retention times, probably as a result of the longer carbon chain length, the lack of a cis double bond, or both, Another difference is that the relative amount of highly unsaturated fatty acid-containing LCOs produced by R. l. by. trifolii is much smaller An isogenic R. l. by. trifolii strain containing a Tn5 insertion in the nodE gene only produces LCOs containing the common fatty acyl groups and not the highly unsaturated types, The results indicate that the difference in the host range of the R. l. bvs, viciae and trifolii is determined by the overall hydrophobicity of the highly unsaturated fatty acyl moieties of LCOs rather than by a specific structural feature.

KW - CHITIN

KW - MASS SPECTROMETRY

KW - SIGNAL MOLECULES

KW - LIPO-OLIGOSACCHARIDE SIGNALS

KW - SYM-PLASMID

KW - ROOT NODULE

KW - GENES

KW - MELILOTI

KW - TRIFOLII

KW - CLOVER

KW - IDENTIFICATION

KW - RECOGNITION

KW - EXPRESSION

M3 - Article

VL - 8

SP - 155

EP - 164

JO - MOLECULAR PLANT-MICROBE INTERACTIONS

JF - MOLECULAR PLANT-MICROBE INTERACTIONS

SN - 0894-0282

IS - 1

ER -