By the same authors

From the same journal

From the same journal

Human cell dedifferentiation in mesenchymal condensates through controlled autophagy

Research output: Contribution to journalArticlepeer-review

Published copy (DOI)



Publication details

JournalScientific Reports
DateE-pub ahead of print - 20 Aug 2015
DatePublished (current) - 2015
Early online date20/08/15
Original languageEnglish


Tissue and whole organ regeneration is a dramatic biological response to injury that occurs across different plant and animal phyla. It frequently requires the dedifferentiation of mature cells to a condensed mesenchymal blastema, from which replacement tissues develop. Human somatic cells cannot regenerate in this way and differentiation is considered irreversible under normal developmental conditions. Here, we sought to establish in vitro conditions to mimic blastema formation by generating different three-dimensional (3D) condensates of human mesenchymal stromal cells (MSCs). We identified specific 3D growth environments that were sufficient to dedifferentiate aged human MSCs to an early mesendoderm-like state with reversal of age-associated cell hypertrophy and restoration of organized tissue regenerating capacity in vivo. An optimal auophagic response was required to promote cytoplasmic remodeling, mitochondrial regression, and a bioenergetic shift from oxidative phosphorylation to anaerobic metabolism. Our evidence suggests that human cell dedifferentiation can be achieved through autonomously controlled autophagic flux.

Discover related content

Find related publications, people, projects, datasets and more using interactive charts.

View graph of relations