By the same authors

From the same journal

From the same journal

From the same journal

Human gut Bacteroidetes can utilize yeast mannan through a selfish mechanism

Research output: Contribution to journalArticle

Published copy (DOI)

Author(s)

  • Fiona Cuskin
  • Elisabeth C Lowe
  • Max J Temple
  • Yanping Zhu
  • Elizabeth A Cameron
  • Nicholas A Pudlo
  • Nathan T Porter
  • Karthik Urs
  • Alan Cartmell
  • Artur Rogowski
  • Brian S Hamilton
  • Rui Chen
  • Thomas J Tolbert
  • Kathleen Piens
  • Debby Bracke
  • Wouter Vervecken
  • Zalihe Hakki
  • Gaetano Speciale
  • Jose L Munōz-Munōz
  • Andrew Day
  • Maria J Peña
  • Richard McLean
  • Alisdair B Boraston
  • Todd Atherly
  • Cherie J Ziemer
  • Spencer J Williams
  • D Wade Abbott
  • Eric C Martens
  • Harry J Gilbert

Department/unit(s)

Publication details

JournalNature
DateE-pub ahead of print - 7 Jan 2015
DatePublished (current) - 8 Jan 2015
Issue number7533
Volume517
Number of pages5
Pages (from-to)165-169
Early online date7/01/15
Original languageEnglish

Abstract

Yeasts, which have been a component of the human diet for at least 7,000 years, possess an elaborate cell wall α-mannan. The influence of yeast mannan on the ecology of the human microbiota is unknown. Here we show that yeast α-mannan is a viable food source for the Gram-negative bacterium Bacteroides thetaiotaomicron, a dominant member of the microbiota. Detailed biochemical analysis and targeted gene disruption studies support a model whereby limited cleavage of α-mannan on the surface generates large oligosaccharides that are subsequently depolymerized to mannose by the action of periplasmic enzymes. Co-culturing studies showed that metabolism of yeast mannan by B. thetaiotaomicron presents a 'selfish' model for the catabolism of this difficult to breakdown polysaccharide. Genomic comparison with B. thetaiotaomicron in conjunction with cell culture studies show that a cohort of highly successful members of the microbiota has evolved to consume sterically-restricted yeast glycans, an adaptation that may reflect the incorporation of eukaryotic microorganisms into the human diet.

Discover related content

Find related publications, people, projects, datasets and more using interactive charts.

View graph of relations