Human papillomavirus type 16 infection of the cervix: a comparison of differing DNA detection modes and the use of monoclonal antibodies against the major capsid protein

C J Lacey, M Wells, R I Macdermott, P E Gibson

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An immuno-peroxidase technique using monoclonal antibodies against the major capsid protein L1 of HPV-16 was compared with dot-blot hybridisation of cervical scrapes and in situ hybridisation of cervical biopsy specimens for HPV-16 DNA. In a series of 20 patients all techniques were specific for HPV-16 infection. Ten patients were positive by dot-blot hybridisation and half of those were positive by in situ hybridisation. Only one of HPV-16 DNA positive cases showed L1 protein expression, apparently shortly after the onset of clinical infection. Whether major capsid protein expression reflects episodes of viral replication deserves further study.
Original languageEnglish
Pages (from-to)87-91
Number of pages5
JournalGenitourinary medicine
Issue number2
Publication statusPublished - 1991


  • Adult
  • Antibodies, Monoclonal
  • Blotting, Southern
  • Capsid
  • Capsid Proteins
  • Cervix Uteri
  • DNA Probes, HPV
  • Female
  • Humans
  • Immunohistochemistry
  • Oncogene Proteins, Viral
  • Papillomaviridae
  • Tumor Virus Infections
  • Uterine Cervical Diseases

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