Human papillomavirus type 16 infection of the cervix: a comparison of differing DNA detection modes and the use of monoclonal antibodies against the major capsid protein

C J Lacey; M Wells; R I Macdermott; P E Gibson

Human papillomavirus type 16 infection of the cervix: a comparison of differing DNA detection modes and the use of monoclonal antibodies against the major capsid protein

C J Lacey, M Wells, R I Macdermott, P E Gibson

The Hull York Medical School

Research output: Contribution to journal › Article › peer-review

Abstract

An immuno-peroxidase technique using monoclonal antibodies against the major capsid protein L1 of HPV-16 was compared with dot-blot hybridisation of cervical scrapes and in situ hybridisation of cervical biopsy specimens for HPV-16 DNA. In a series of 20 patients all techniques were specific for HPV-16 infection. Ten patients were positive by dot-blot hybridisation and half of those were positive by in situ hybridisation. Only one of HPV-16 DNA positive cases showed L1 protein expression, apparently shortly after the onset of clinical infection. Whether major capsid protein expression reflects episodes of viral replication deserves further study.

Original language	English
Pages (from-to)	87-91
Number of pages	5
Journal	Genitourinary medicine
Volume	67
Issue number	2
Publication status	Published - 1991

Keywords

Adult
Antibodies, Monoclonal
Blotting, Southern
Capsid
Capsid Proteins
Cervix Uteri
DNA Probes, HPV
Female
Humans
Immunohistochemistry
Oncogene Proteins, Viral
Papillomaviridae
Tumor Virus Infections
Uterine Cervical Diseases

Cite this

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title = "Human papillomavirus type 16 infection of the cervix: a comparison of differing DNA detection modes and the use of monoclonal antibodies against the major capsid protein",

abstract = "An immuno-peroxidase technique using monoclonal antibodies against the major capsid protein L1 of HPV-16 was compared with dot-blot hybridisation of cervical scrapes and in situ hybridisation of cervical biopsy specimens for HPV-16 DNA. In a series of 20 patients all techniques were specific for HPV-16 infection. Ten patients were positive by dot-blot hybridisation and half of those were positive by in situ hybridisation. Only one of HPV-16 DNA positive cases showed L1 protein expression, apparently shortly after the onset of clinical infection. Whether major capsid protein expression reflects episodes of viral replication deserves further study.",

keywords = "Adult, Antibodies, Monoclonal, Blotting, Southern, Capsid, Capsid Proteins, Cervix Uteri, DNA Probes, HPV, Female, Humans, Immunohistochemistry, Oncogene Proteins, Viral, Papillomaviridae, Tumor Virus Infections, Uterine Cervical Diseases",

author = "Lacey, {C J} and M Wells and Macdermott, {R I} and Gibson, {P E}",

year = "1991",

language = "English",

volume = "67",

pages = "87--91",

journal = "Genitourinary medicine",

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T1 - Human papillomavirus type 16 infection of the cervix

T2 - a comparison of differing DNA detection modes and the use of monoclonal antibodies against the major capsid protein

AU - Lacey, C J

AU - Wells, M

AU - Macdermott, R I

AU - Gibson, P E

PY - 1991

Y1 - 1991

N2 - An immuno-peroxidase technique using monoclonal antibodies against the major capsid protein L1 of HPV-16 was compared with dot-blot hybridisation of cervical scrapes and in situ hybridisation of cervical biopsy specimens for HPV-16 DNA. In a series of 20 patients all techniques were specific for HPV-16 infection. Ten patients were positive by dot-blot hybridisation and half of those were positive by in situ hybridisation. Only one of HPV-16 DNA positive cases showed L1 protein expression, apparently shortly after the onset of clinical infection. Whether major capsid protein expression reflects episodes of viral replication deserves further study.

AB - An immuno-peroxidase technique using monoclonal antibodies against the major capsid protein L1 of HPV-16 was compared with dot-blot hybridisation of cervical scrapes and in situ hybridisation of cervical biopsy specimens for HPV-16 DNA. In a series of 20 patients all techniques were specific for HPV-16 infection. Ten patients were positive by dot-blot hybridisation and half of those were positive by in situ hybridisation. Only one of HPV-16 DNA positive cases showed L1 protein expression, apparently shortly after the onset of clinical infection. Whether major capsid protein expression reflects episodes of viral replication deserves further study.

KW - Adult

KW - Antibodies, Monoclonal

KW - Blotting, Southern

KW - Capsid

KW - Capsid Proteins

KW - Cervix Uteri

KW - DNA Probes, HPV

KW - Female

KW - Humans

KW - Immunohistochemistry

KW - Oncogene Proteins, Viral

KW - Papillomaviridae

KW - Tumor Virus Infections

KW - Uterine Cervical Diseases

M3 - Article

C2 - 1851722

SN - 0266-4348

VL - 67

SP - 87

EP - 91

JO - Genitourinary medicine

JF - Genitourinary medicine

IS - 2

ER -