Human sperm swimming in a high viscosity mucus analogue

TY - JOUR

T1 - Human sperm swimming in a high viscosity mucus analogue

AU - Ishimoto, Kenta

AU - Gadelha, Hermes Augusto Buarque

AU - Gaffney, Eamonn A.

AU - Smith, David

AU - Kirkman-Brown, Jackson

PY - 2018/3/9

Y1 - 2018/3/9

N2 - Remarkably, mammalian sperm maintain a substantive proportion of their progressive swimming speed within highly viscous fluids, including those of the female reproductive tract. Here, we analyse the digital microscopy of a human sperm swimming in a highly viscous, weakly elastic mucus analogue. We exploit principal component analysis to simplify its flagellar beat pattern, from which boundary element calculations are used to determine the time-dependent flow field around the sperm cell. The sperm flow field is further approximated in terms of regularized point forces, and estimates of the mechanical power consumption are determined, for comparison with analogous low viscosity media studies. This highlights extensive differences in the structure of the flows surrounding human sperm in different media, indicating how the cell-cell and cell- boundary hydrodynamic interactions significantly differ with the physical microenvironment. The regularized point force decomposition also provides cell-level information that may ulti- mately be incorporated into sperm population models. We further observe indications that the core feature in explaining the effectiveness of sperm swimming in high viscosity media is the loss of cell yawing, which is related with a greater density of regularized point force singularities along the axis of symmetry of the flagellar beat to represent the flow field. In turn this implicates a reduction of the wavelength of the distal beat pattern – and hence dynamical wavelength selection of the flagellar beat – as the dominant feature governing the effectiveness of sperm swimming in highly viscous media.

AB - Remarkably, mammalian sperm maintain a substantive proportion of their progressive swimming speed within highly viscous fluids, including those of the female reproductive tract. Here, we analyse the digital microscopy of a human sperm swimming in a highly viscous, weakly elastic mucus analogue. We exploit principal component analysis to simplify its flagellar beat pattern, from which boundary element calculations are used to determine the time-dependent flow field around the sperm cell. The sperm flow field is further approximated in terms of regularized point forces, and estimates of the mechanical power consumption are determined, for comparison with analogous low viscosity media studies. This highlights extensive differences in the structure of the flows surrounding human sperm in different media, indicating how the cell-cell and cell- boundary hydrodynamic interactions significantly differ with the physical microenvironment. The regularized point force decomposition also provides cell-level information that may ulti- mately be incorporated into sperm population models. We further observe indications that the core feature in explaining the effectiveness of sperm swimming in high viscosity media is the loss of cell yawing, which is related with a greater density of regularized point force singularities along the axis of symmetry of the flagellar beat to represent the flow field. In turn this implicates a reduction of the wavelength of the distal beat pattern – and hence dynamical wavelength selection of the flagellar beat – as the dominant feature governing the effectiveness of sperm swimming in highly viscous media.

U2 - 10.1016/j.jtbi.2018.02.013

DO - 10.1016/j.jtbi.2018.02.013

M3 - Article

SN - 0022-5193

VL - 446

SP - 1

EP - 10

JO - Journal of theoretical biology

JF - Journal of theoretical biology

ER -