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Seven slow-growing rhizobia isolated from effective nodules of Arachis hypogaea were assigned to the genus Bradyrhizobium by sharing 96.3-99.9% similarities of 16S rRNA gene sequences with the recognized Bradyrhizobium species. Multilocus sequence analysis of glnII, recA, gyrB and dnaK genes indicated that the seven strains belonged to two novel species represented by CCBAU 51649T and CCBAU 53363T. Strain CCBAU 51649T had 94%, 93.4%, 92.3% and 94.9% and CCBAU 53363T had 91.4%, 94.5%, 94.6% and 97.7% sequences similarities for glnII, recA, gyrB and dnaK genes with respect to the closest related species Bradyrhizobium manausense BR3351T or Bradyrhizobium yuanmingense CCBAU 10071T, respectively. Summed feature 8 and C16:0 were the predominant components for strains CCBAU 51649T and CCBAU 53363T. DNA-DNA hybridization and analysis of phenotypic characteristics also distinguished these strains from the closest related Bradyrhizobium species. The two species formed effective nodules in Arachis hypogaea, Lablab purpureus and Aeschynomene indica, and they have identical nodA to strain PJI237 but are phylogenetically divergent to other available nodA genes with less than 66% similarity. Based in these results, Bradyrhizobium guangdongense sp. nov. and Bradyrhizobium guangxiense sp. nov. are proposed, and CCBAU 51649T (=CGMCC 1.15034T =LMG 28620T) and CCBAU 53363T (=CGMCC 1.15035T =LMG 28621T) are designated as the type strains, respectively.
|Journal||International Journal of Systematic and Evolutionary Microbiology|
|Early online date||23 Sept 2015|
|Publication status||Published - 2015|