Identification and biochemical characterization of an Arabidopsis indole-3-acetic acid glucosyltransferase

R G Jackson; E K Lim; Y Li; M Kowalczyk; G Sandberg; J Hoggett; D A Ashford; D J Bowles

Identification and biochemical characterization of an Arabidopsis indole-3-acetic acid glucosyltransferase

R G Jackson, E K Lim, Y Li, M Kowalczyk, G Sandberg, J Hoggett, D A Ashford, D J Bowles

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Abstract

Biochemical characterization of recombinant gene products following a phylogenetic analysis of the UDP-glucosyltransferase (UGT) multigene family of Arabidopsis has identified one enzyme (UGT84B1) with high activity toward the plant hormone indole-3-acetic acid (IAA) and three related enzymes (UGT84B2, UGT75B1, and UGT75B2) with trace activities. The identity of the IAA conjugate has been confirmed to be 1-O-indole acetyl glucose ester. A sequence annotated as a UDP-glucose:IAA glucosyltransferase (IAA-UGT) in the Arabidopsis genome and expressed sequence tag data bases given its similarity to the maize iaglu gene sequence showed no activity toward IAA This study describes the first biochemical analysis of a recombinant IAA-UGT and provides the foundation for future genetic approaches to understand the role of 1-O-indole acetyl glucose ester in Arabidopsis.

Original language	English
Pages (from-to)	4350-4356
Number of pages	7
Journal	Journal of Biological Chemistry
Volume	276
Issue number	6
Publication status	Published - 9 Feb 2001

Keywords

BETA-D-GLUCOSYL
ZEA-MAYS-L
ENZYMATIC-SYNTHESIS
ENZYMIC-SYNTHESIS
O-GLUCOSYLTRANSFERASE
LIQUID-CHROMATOGRAPHY
PHASEOLUS-LUNATUS
METABOLISM
GENE
INDOL-3-YLACETYL-MYO-INOSITOL

Cite this

@article{cdce8e786d284b5da5430c1e784bfa7c,

title = "Identification and biochemical characterization of an Arabidopsis indole-3-acetic acid glucosyltransferase",

abstract = "Biochemical characterization of recombinant gene products following a phylogenetic analysis of the UDP-glucosyltransferase (UGT) multigene family of Arabidopsis has identified one enzyme (UGT84B1) with high activity toward the plant hormone indole-3-acetic acid (IAA) and three related enzymes (UGT84B2, UGT75B1, and UGT75B2) with trace activities. The identity of the IAA conjugate has been confirmed to be 1-O-indole acetyl glucose ester. A sequence annotated as a UDP-glucose:IAA glucosyltransferase (IAA-UGT) in the Arabidopsis genome and expressed sequence tag data bases given its similarity to the maize iaglu gene sequence showed no activity toward IAA This study describes the first biochemical analysis of a recombinant IAA-UGT and provides the foundation for future genetic approaches to understand the role of 1-O-indole acetyl glucose ester in Arabidopsis.",

keywords = "BETA-D-GLUCOSYL, ZEA-MAYS-L, ENZYMATIC-SYNTHESIS, ENZYMIC-SYNTHESIS, O-GLUCOSYLTRANSFERASE, LIQUID-CHROMATOGRAPHY, PHASEOLUS-LUNATUS, METABOLISM, GENE, INDOL-3-YLACETYL-MYO-INOSITOL",

author = "Jackson, {R G} and Lim, {E K} and Y Li and M Kowalczyk and G Sandberg and J Hoggett and Ashford, {D A} and Bowles, {D J}",

year = "2001",

month = feb,

day = "9",

language = "English",

volume = "276",

pages = "4350--4356",

journal = "Journal of Biological Chemistry",

issn = "0021-9258",

publisher = "American Society for Biochemistry and Molecular Biology Inc.",

number = "6",

}

TY - JOUR

T1 - Identification and biochemical characterization of an Arabidopsis indole-3-acetic acid glucosyltransferase

AU - Jackson, R G

AU - Lim, E K

AU - Li, Y

AU - Kowalczyk, M

AU - Sandberg, G

AU - Hoggett, J

AU - Ashford, D A

AU - Bowles, D J

PY - 2001/2/9

Y1 - 2001/2/9

N2 - Biochemical characterization of recombinant gene products following a phylogenetic analysis of the UDP-glucosyltransferase (UGT) multigene family of Arabidopsis has identified one enzyme (UGT84B1) with high activity toward the plant hormone indole-3-acetic acid (IAA) and three related enzymes (UGT84B2, UGT75B1, and UGT75B2) with trace activities. The identity of the IAA conjugate has been confirmed to be 1-O-indole acetyl glucose ester. A sequence annotated as a UDP-glucose:IAA glucosyltransferase (IAA-UGT) in the Arabidopsis genome and expressed sequence tag data bases given its similarity to the maize iaglu gene sequence showed no activity toward IAA This study describes the first biochemical analysis of a recombinant IAA-UGT and provides the foundation for future genetic approaches to understand the role of 1-O-indole acetyl glucose ester in Arabidopsis.

AB - Biochemical characterization of recombinant gene products following a phylogenetic analysis of the UDP-glucosyltransferase (UGT) multigene family of Arabidopsis has identified one enzyme (UGT84B1) with high activity toward the plant hormone indole-3-acetic acid (IAA) and three related enzymes (UGT84B2, UGT75B1, and UGT75B2) with trace activities. The identity of the IAA conjugate has been confirmed to be 1-O-indole acetyl glucose ester. A sequence annotated as a UDP-glucose:IAA glucosyltransferase (IAA-UGT) in the Arabidopsis genome and expressed sequence tag data bases given its similarity to the maize iaglu gene sequence showed no activity toward IAA This study describes the first biochemical analysis of a recombinant IAA-UGT and provides the foundation for future genetic approaches to understand the role of 1-O-indole acetyl glucose ester in Arabidopsis.

KW - BETA-D-GLUCOSYL

KW - ZEA-MAYS-L

KW - ENZYMATIC-SYNTHESIS

KW - ENZYMIC-SYNTHESIS

KW - O-GLUCOSYLTRANSFERASE

KW - LIQUID-CHROMATOGRAPHY

KW - PHASEOLUS-LUNATUS

KW - METABOLISM

KW - GENE

KW - INDOL-3-YLACETYL-MYO-INOSITOL

M3 - Article

SN - 0021-9258

VL - 276

SP - 4350

EP - 4356

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 6

ER -