Identification and characterisation of Arabidopsis glycosyltransferases capable of glucosylating coniferyl aldehyde and sinapyl aldehyde

E K Lim; R G Jackson; D J Bowles

doi:10.1016/j.febslet.2005.04.016

Identification and characterisation of Arabidopsis glycosyltransferases capable of glucosylating coniferyl aldehyde and sinapyl aldehyde

E K Lim, R G Jackson, D J Bowles

CNAP

Research output: Contribution to journal › Article › peer-review

Abstract

This study describes the substrate recognition profile of UGT72E1, an UDP-glucose:glycosyltransferase of Arabidopsis thaliana that is the third member of a branch of glycosyltransferases, capable of conjugating lignin monomers and related metabolites. The data show that UGT72E1, in contrast to the two closely related UGTs 72E2 and 72E3, is specific for sinapyl and coniferyl aldehydes. The biochemical properties of UGT72E1 are characterised, and are compared with that of UGT72E2, which is capable of glycosylating the aldehydes as well as coniferyl and sinapyl alcohols. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	2802-2806
Number of pages	5
Journal	FEBS Letters
Volume	579
Issue number	13
DOIs	https://doi.org/10.1016/j.febslet.2005.04.016
Publication status	Published - 23 May 2005

Keywords

glycosyltransferase
lignin
monolignol
coniferyl aldehyde
sinapyl aldehyde
ALCOHOL GLUCOSYLTRANSFERASE
LIGNIN BIOSYNTHESIS
METABOLISM
THALIANA
XYLEM

Access to Document

10.1016/j.febslet.2005.04.016

Glucosyltransferases of the common phenylpropanoid pathway
Bowles, D. J. (Principal investigator)
BBSRC
1/10/04 → 30/09/07
Project: Research project (funded) › Research

Cite this

@article{05b88d8e68dd4703b8d9f91b41d77167,

title = "Identification and characterisation of Arabidopsis glycosyltransferases capable of glucosylating coniferyl aldehyde and sinapyl aldehyde",

abstract = "This study describes the substrate recognition profile of UGT72E1, an UDP-glucose:glycosyltransferase of Arabidopsis thaliana that is the third member of a branch of glycosyltransferases, capable of conjugating lignin monomers and related metabolites. The data show that UGT72E1, in contrast to the two closely related UGTs 72E2 and 72E3, is specific for sinapyl and coniferyl aldehydes. The biochemical properties of UGT72E1 are characterised, and are compared with that of UGT72E2, which is capable of glycosylating the aldehydes as well as coniferyl and sinapyl alcohols. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.",

keywords = "glycosyltransferase, lignin, monolignol, coniferyl aldehyde, sinapyl aldehyde, ALCOHOL GLUCOSYLTRANSFERASE, LIGNIN BIOSYNTHESIS, METABOLISM, THALIANA, XYLEM",

author = "Lim, {E K} and Jackson, {R G} and Bowles, {D J}",

year = "2005",

month = may,

day = "23",

doi = "10.1016/j.febslet.2005.04.016",

language = "English",

volume = "579",

pages = "2802--2806",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Wiley-Blackwell",

number = "13",

}

TY - JOUR

T1 - Identification and characterisation of Arabidopsis glycosyltransferases capable of glucosylating coniferyl aldehyde and sinapyl aldehyde

AU - Lim, E K

AU - Jackson, R G

AU - Bowles, D J

PY - 2005/5/23

Y1 - 2005/5/23

N2 - This study describes the substrate recognition profile of UGT72E1, an UDP-glucose:glycosyltransferase of Arabidopsis thaliana that is the third member of a branch of glycosyltransferases, capable of conjugating lignin monomers and related metabolites. The data show that UGT72E1, in contrast to the two closely related UGTs 72E2 and 72E3, is specific for sinapyl and coniferyl aldehydes. The biochemical properties of UGT72E1 are characterised, and are compared with that of UGT72E2, which is capable of glycosylating the aldehydes as well as coniferyl and sinapyl alcohols. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

AB - This study describes the substrate recognition profile of UGT72E1, an UDP-glucose:glycosyltransferase of Arabidopsis thaliana that is the third member of a branch of glycosyltransferases, capable of conjugating lignin monomers and related metabolites. The data show that UGT72E1, in contrast to the two closely related UGTs 72E2 and 72E3, is specific for sinapyl and coniferyl aldehydes. The biochemical properties of UGT72E1 are characterised, and are compared with that of UGT72E2, which is capable of glycosylating the aldehydes as well as coniferyl and sinapyl alcohols. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

KW - glycosyltransferase

KW - lignin

KW - monolignol

KW - coniferyl aldehyde

KW - sinapyl aldehyde

KW - ALCOHOL GLUCOSYLTRANSFERASE

KW - LIGNIN BIOSYNTHESIS

KW - METABOLISM

KW - THALIANA

KW - XYLEM

U2 - 10.1016/j.febslet.2005.04.016

DO - 10.1016/j.febslet.2005.04.016

M3 - Article

SN - 0014-5793

VL - 579

SP - 2802

EP - 2806

JO - FEBS Letters

JF - FEBS Letters

IS - 13

ER -

Identification and characterisation of Arabidopsis glycosyltransferases capable of glucosylating coniferyl aldehyde and sinapyl aldehyde

Abstract

Keywords

Access to Document

Projects

Glucosyltransferases of the common phenylpropanoid pathway

Cite this