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Identification of a fatty acid Delta 11-desaturase from the microalga Thalassiosira pseudonana

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JournalFEBS Letters
DatePublished - 9 Apr 2004
Issue number1-3
Volume563
Number of pages7
Pages (from-to)28-34
Original languageEnglish

Abstract

A set of genomic DNA sequences putatively encoding front-end desaturases were identified by in silico analysis of the draft genome of the marine microalga Thalassiosira pseudonana. Among these candidate genes, an open reading frame named TpdesN was found to be full-length, intronless, and constitutively expressed during cell cultivation. The predicted amino acid sequence of the corresponding protein, TpDESN, exhibited typical features of desaturases involved in the production of polyunsaturated fatty acids (PUFAs) in algae, i.e. a cytochrome b5-like domain at the N-terminus and three conserved histidine-rich motifs in the desaturase domain. Expression of TpDESN in Saccharomyces cerevisiae revealed that this enzyme was not involved in PUFA synthesis, but specifically desaturated palmitic acid 16:0 to 16:1Delta11. To our knowledge, until this report, Delta11-desaturase activity had only been detected in insect cells. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

    Research areas

  • yeast expression, Delta 11-desaturase, Thalassiosira pseudonana, DOUBLE-BOND POSITION, SACCHAROMYCES-CEREVISIAE, EICOSAPENTAENOIC ACID, DOCOSAHEXAENOIC ACID, DESATURASE GENE, BIOSYNTHESIS, EVOLUTION, PHEROMONES, PROTEINS, PAVLOVA

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