Identification of a very long chain polyunsaturated fatty acid Δ4-desaturase from the microalga Pavlova lutheri

Thierry Tonon; David Harvey; Tony R. Larson; Ian A. Graham

doi:10.1016/S0014-5793(03)01078-0

Identification of a very long chain polyunsaturated fatty acid Δ4-desaturase from the microalga Pavlova lutheri

Thierry Tonon, David Harvey, Tony R. Larson, Ian A. Graham^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Pavlova lutheri, a marine microalga, is rich in the very long chain polyunsaturated fatty acids (VLCPUFAs) eicosapentaenoic (20:5n-3) and docosahexaenoic (22:6n-3) acids. Using an expressed sequence tag approach, we isolated a cDNA designated Pldes1, and encoding an amino acid sequence showing high similarity with polyunsaturated fatty acid front-end desaturases. Heterologous expression in yeast demonstrated that PlDES1 desaturated 22:5n-3 and 22:4n-6 into 22:6n-3 and 22:5n-6 respectively, and was equally active on both substrates. Thus, PlDES1 is a novel VLCPUFA Δ4-desaturase. Pldes1 expression is four-fold higher during the mid-exponential phase of growth compared to late exponential and stationary phases.

Original language	English
Pages (from-to)	440-444
Number of pages	5
Journal	FEBS Letters
Volume	553
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(03)01078-0
Publication status	Published - 23 Oct 2003

Keywords

Docosahexaenoic acid
Pavlova lutheri
Very long chain polyunsaturated fatty acid
Δ4-Desaturase

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title = "Identification of a very long chain polyunsaturated fatty acid Δ4-desaturase from the microalga Pavlova lutheri",

abstract = "Pavlova lutheri, a marine microalga, is rich in the very long chain polyunsaturated fatty acids (VLCPUFAs) eicosapentaenoic (20:5n-3) and docosahexaenoic (22:6n-3) acids. Using an expressed sequence tag approach, we isolated a cDNA designated Pldes1, and encoding an amino acid sequence showing high similarity with polyunsaturated fatty acid front-end desaturases. Heterologous expression in yeast demonstrated that PlDES1 desaturated 22:5n-3 and 22:4n-6 into 22:6n-3 and 22:5n-6 respectively, and was equally active on both substrates. Thus, PlDES1 is a novel VLCPUFA Δ4-desaturase. Pldes1 expression is four-fold higher during the mid-exponential phase of growth compared to late exponential and stationary phases.",

keywords = "Docosahexaenoic acid, Pavlova lutheri, Very long chain polyunsaturated fatty acid, Δ4-Desaturase",

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doi = "10.1016/S0014-5793(03)01078-0",

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T1 - Identification of a very long chain polyunsaturated fatty acid Δ4-desaturase from the microalga Pavlova lutheri

AU - Tonon, Thierry

AU - Harvey, David

AU - Larson, Tony R.

AU - Graham, Ian A.

PY - 2003/10/23

Y1 - 2003/10/23

N2 - Pavlova lutheri, a marine microalga, is rich in the very long chain polyunsaturated fatty acids (VLCPUFAs) eicosapentaenoic (20:5n-3) and docosahexaenoic (22:6n-3) acids. Using an expressed sequence tag approach, we isolated a cDNA designated Pldes1, and encoding an amino acid sequence showing high similarity with polyunsaturated fatty acid front-end desaturases. Heterologous expression in yeast demonstrated that PlDES1 desaturated 22:5n-3 and 22:4n-6 into 22:6n-3 and 22:5n-6 respectively, and was equally active on both substrates. Thus, PlDES1 is a novel VLCPUFA Δ4-desaturase. Pldes1 expression is four-fold higher during the mid-exponential phase of growth compared to late exponential and stationary phases.

AB - Pavlova lutheri, a marine microalga, is rich in the very long chain polyunsaturated fatty acids (VLCPUFAs) eicosapentaenoic (20:5n-3) and docosahexaenoic (22:6n-3) acids. Using an expressed sequence tag approach, we isolated a cDNA designated Pldes1, and encoding an amino acid sequence showing high similarity with polyunsaturated fatty acid front-end desaturases. Heterologous expression in yeast demonstrated that PlDES1 desaturated 22:5n-3 and 22:4n-6 into 22:6n-3 and 22:5n-6 respectively, and was equally active on both substrates. Thus, PlDES1 is a novel VLCPUFA Δ4-desaturase. Pldes1 expression is four-fold higher during the mid-exponential phase of growth compared to late exponential and stationary phases.

KW - Docosahexaenoic acid

KW - Pavlova lutheri

KW - Very long chain polyunsaturated fatty acid

KW - Δ4-Desaturase

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DO - 10.1016/S0014-5793(03)01078-0

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C2 - 14572666

AN - SCOPUS:0142231412

SN - 0014-5793

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EP - 444

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

Identification of a very long chain polyunsaturated fatty acid Δ4-desaturase from the microalga Pavlova lutheri

Abstract

Keywords

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