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Identification of a very long chain polyunsaturated fatty acid Delta4-desaturase from the microalga Pavlova lutheri

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JournalFEBS Letters
DatePublished - 23 Oct 2003
Issue number3
Volume553
Number of pages5
Pages (from-to)440-4
Original languageEnglish

Abstract

Pavlova lutheri, a marine microalga, is rich in the very long chain polyunsaturated fatty acids (VLCPUFAs) eicosapentaenoic (20:5n-3) and docosahexaenoic (22:6n-3) acids. Using an expressed sequence tag approach, we isolated a cDNA designated Pldes1, and encoding an amino acid sequence showing high similarity with polyunsaturated fatty acid front-end desaturases. Heterologous expression in yeast demonstrated that PlDES1 desaturated 22:5n-3 and 22:4n-6 into 22:6n-3 and 22:5n-6 respectively, and was equally active on both substrates. Thus, PlDES1 is a novel VLCPUFA Delta4-desaturase. Pldes1 expression is four-fold higher during the mid-exponential phase of growth compared to late exponential and stationary phases.

    Research areas

  • Amino Acid Sequence, Chromatography, Gas, DNA, Complementary/genetics, Docosahexaenoic Acids/metabolism, Eicosapentaenoic Acid/analogs & derivatives, Eukaryota/chemistry, Fatty Acid Desaturases/chemistry, Fatty Acids, Unsaturated/analysis, Gene Expression Regulation, Enzymologic/physiology, Molecular Sequence Data, Recombinant Proteins/chemistry, Sequence Homology, Amino Acid, Yeasts/genetics

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