Identification of glucosyltransferase genes involved in sinapate metabolism and lignin synthesis in Arabidopsis

E K Lim, Y Li, A Parr, R Jackson, D A Ashford, D J Bowles

Research output: Contribution to journalArticlepeer-review

Abstract

Sinapic acid is a major phenylpropanoid in Brassicaceae providing intermediates in two distinct metabolic pathways leading to sinapoyl esters and lignin synthesis. Glucosyltransferases play key roles in the formation of these intermediates, either through the production of the high energy compound 1-O-sinapoyl-glucose leading to sinapoylmalate and sinapoylcholine or through the production of sinapyl alcohol-4-O-glucoside, potentially leading to the syringyl units found in lignins. While the importance of these glucosyltransferases has been recognized for more than 20 years, their corresponding genes have not been identified. Combining sequence information in the Arabidopsis genomic data base with biochemical data from screening the activity of recombinant proteins in vitro, we have now identified five gene sequences encoding enzymes that can glucosylate sinapic acid, sinapyl alcohol, and their related phenylpropanoids. The data provide a foundation for future understanding and manipulation of sinapate metabolism and lignin biology in Arabidopsis.

Original languageEnglish
Pages (from-to)4344-4349
Number of pages6
JournalJournal of Biological Chemistry
Volume276
Issue number6
Publication statusPublished - 9 Feb 2001

Keywords

  • UDP-GLUCOSE GLUCOSYLTRANSFERASE
  • BIOCHEMICAL-CHARACTERIZATION
  • O-GLUCOSYLTRANSFERASE
  • RAPHANUS-SATIVUS
  • SALICYLIC-ACID
  • EXPRESSION
  • CLONING
  • BIOSYNTHESIS
  • COTYLEDONS
  • 5-O-GLUCOSYLTRANSFERASE

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