Identification of novel esterases for the synthesis of sterically demanding chiral alcohols by sequence-structure guided genome mining

G.-S. Nguyen, M.L. Thompson, U.T. Bornscheuer, R. Kourist, G. Grogan

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Six esterases isolated from sequence and structure-guided genome mining approaches were evaluated for the kinetic resolution of secondary and tertiary alcohols that find application in the fine chemical and pharmaceutical industries. Activity and enantioselectivity with E-values of up to 24 were determined towards a range of sterically demanding tertiary alcohol esters. Excellent enantioselectivity (E > 100) was also achieved in the hydrolysis of a less challenging secondary alcohol ester, menthyl acetate. These results highlight that these approaches can be used for the identification of novel esterases applicable to the preparation of commercially desirable alcohols.
Original languageEnglish
Pages (from-to)88-94
Number of pages7
JournalJournal of Molecular Catalysis B : Enzymatic
Issue number3-4
Publication statusPublished - 1 Jul 2011

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