IL-18 potentiates the adjuvant properties of IL-12 in the induction of a strong Th1 type immune response against a recombinant antigen

Matthias Eberl; E Beck; P S Coulson; H Okamura; R A Wilson; A P Mountford

IL-18 potentiates the adjuvant properties of IL-12 in the induction of a strong Th1 type immune response against a recombinant antigen

Matthias Eberl, E Beck, P S Coulson, H Okamura, R A Wilson, A P Mountford

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Due to the synergistic effects of IL-12 and IL-18, and to their importance in establishing a Th1 type immune response, we investigated the potential of a combined administration of both cytokines as an adjuvant for recombinant antigens. As a model system, we used a schistosome T cell antigen recently identified in our group. By co-adsorption of this antigen on alum in the presence of IL-12 and IL-18, we demonstrate that IL-18 enhances the effects of IL-12 in inducing an antigen-specific Th1 type CD4(+) T cell response as well as high titres of IgG1, IgG2a, and IgG2b antibodies.

Original language	English
Pages (from-to)	2002-8
Number of pages	7
Journal	Vaccine
Volume	18
Issue number	19
Publication status	Published - 2000

Keywords

Adjuvants, Immunologic
Animals
Antibodies, Helminth
Antigens
Antigens, Helminth
Drug Synergism
Female
Helminth Proteins
Immunoglobulin G
Interleukin-12
Interleukin-18
Mice
Mice, Inbred C57BL
Recombinant Proteins
Schistosoma mansoni
Th1 Cells

Cite this

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title = "IL-18 potentiates the adjuvant properties of IL-12 in the induction of a strong Th1 type immune response against a recombinant antigen",

abstract = "Due to the synergistic effects of IL-12 and IL-18, and to their importance in establishing a Th1 type immune response, we investigated the potential of a combined administration of both cytokines as an adjuvant for recombinant antigens. As a model system, we used a schistosome T cell antigen recently identified in our group. By co-adsorption of this antigen on alum in the presence of IL-12 and IL-18, we demonstrate that IL-18 enhances the effects of IL-12 in inducing an antigen-specific Th1 type CD4(+) T cell response as well as high titres of IgG1, IgG2a, and IgG2b antibodies.",

keywords = "Adjuvants, Immunologic, Animals, Antibodies, Helminth, Antigens, Antigens, Helminth, Drug Synergism, Female, Helminth Proteins, Immunoglobulin G, Interleukin-12, Interleukin-18, Mice, Mice, Inbred C57BL, Recombinant Proteins, Schistosoma mansoni, Th1 Cells",

author = "Matthias Eberl and E Beck and Coulson, {P S} and H Okamura and Wilson, {R A} and Mountford, {A P}",

year = "2000",

language = "English",

volume = "18",

pages = "2002--8",

journal = "Vaccine",

issn = "0264-410X",

publisher = "Elsevier",

number = "19",

}

TY - JOUR

T1 - IL-18 potentiates the adjuvant properties of IL-12 in the induction of a strong Th1 type immune response against a recombinant antigen

AU - Eberl, Matthias

AU - Beck, E

AU - Coulson, P S

AU - Okamura, H

AU - Wilson, R A

AU - Mountford, A P

PY - 2000

Y1 - 2000

N2 - Due to the synergistic effects of IL-12 and IL-18, and to their importance in establishing a Th1 type immune response, we investigated the potential of a combined administration of both cytokines as an adjuvant for recombinant antigens. As a model system, we used a schistosome T cell antigen recently identified in our group. By co-adsorption of this antigen on alum in the presence of IL-12 and IL-18, we demonstrate that IL-18 enhances the effects of IL-12 in inducing an antigen-specific Th1 type CD4(+) T cell response as well as high titres of IgG1, IgG2a, and IgG2b antibodies.

AB - Due to the synergistic effects of IL-12 and IL-18, and to their importance in establishing a Th1 type immune response, we investigated the potential of a combined administration of both cytokines as an adjuvant for recombinant antigens. As a model system, we used a schistosome T cell antigen recently identified in our group. By co-adsorption of this antigen on alum in the presence of IL-12 and IL-18, we demonstrate that IL-18 enhances the effects of IL-12 in inducing an antigen-specific Th1 type CD4(+) T cell response as well as high titres of IgG1, IgG2a, and IgG2b antibodies.

KW - Adjuvants, Immunologic

KW - Animals

KW - Antibodies, Helminth

KW - Antigens

KW - Antigens, Helminth

KW - Drug Synergism

KW - Female

KW - Helminth Proteins

KW - Immunoglobulin G

KW - Interleukin-12

KW - Interleukin-18

KW - Mice

KW - Mice, Inbred C57BL

KW - Recombinant Proteins

KW - Schistosoma mansoni

KW - Th1 Cells

M3 - Article

C2 - 10706961

VL - 18

SP - 2002

EP - 2008

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 19

ER -